Department of Radiotherapy, Chair of Oncology, Medical University of Lodz, Poland.
Department of Nephropathology, Division of Morphometry, Medical University of Lodz, Poland.
Arch Med Sci. 2014 Feb 24;10(1):84-90. doi: 10.5114/aoms.2013.36753. Epub 2013 Aug 12.
The value of argyrophilic nucleolar organizer regions (AgNORs) to predict survival in patients with ovarian cancer has not been clearly explained yet. The aim of study was to assess the value of analysis of the mean number of AgNORs per nucleus (mAgNOR) and mean percentage of nuclei with five or more AgNORs per nucleus (pAgNOR) in the prediction of disease-free survival (DFS) and overall survival (OS) in patients with serous ovarian cancer.
The study examined 52 patients treated for serous ovarian cancer with a follow-up period of 2-143 months. After silver staining paraffin specimens from primary surgery, mAgNOR and pAgNOR in cancer cells were counted and analyzed. Age, grading, radicality of surgery and FIGO staging were analyzed as covariates.
Mean mAgNOR equaled 4.4 ±0.9 and pAgNOR equaled 42.2 ±20.8%. Both mAgNOR and pAgNOR were the lowest in G1 tumors. The mAgNOR and pAgNOR were lower in stage I than stage IV cancers. The DFS and OS rates were respectively 15.4% and 21.2%. In univariate analysis FIGO staging, grading, and pAgNOR were associated with worse prognosis, while radicality of surgery remained a significant protective factor in terms of DFS. Higher FIGO staging and older age worsened OS. In multivariate analysis FIGO staging remained significantly associated with both DFS (HR 1.98; 95% CI 1.05-3.71) and OS (HR 1.76; 95% CI 1.00-3.10), while age affected OS rates (HR 1.78; 95% CI 1.04-2.95).
mAgNOR and pAgNOR are useful markers of cellular kinetics. Prospective studies in larger populations are needed to confirm these results in terms of AgNORs' effects on survival.
银染核仁组成区相关嗜银蛋白（AgNORs）在预测卵巢癌患者生存方面的价值尚未得到明确解释。本研究旨在评估核仁组成区相关嗜银蛋白平均核计数（mAgNOR）和每核 5 个或更多核仁组成区相关嗜银蛋白核的比例（pAgNOR）分析在预测浆液性卵巢癌患者无病生存（DFS）和总生存（OS）方面的价值。
本研究共纳入 52 例接受浆液性卵巢癌治疗的患者，随访时间为 2-143 个月。对初次手术的石蜡标本进行银染后，计数并分析癌细胞中的 mAgNOR 和 pAgNOR。分析年龄、分级、手术根治性和 FIGO 分期作为协变量。
mAgNOR 平均值为 4.4 ±0.9，pAgNOR 平均值为 42.2 ±20.8%。G1 肿瘤的 mAgNOR 和 pAgNOR 均最低。I 期癌症的 mAgNOR 和 pAgNOR 低于 IV 期癌症。DFS 和 OS 率分别为 15.4%和 21.2%。单因素分析显示，FIGO 分期、分级和 pAgNOR 与预后不良相关，而手术根治性仍然是 DFS 的显著保护因素。较高的 FIGO 分期和年龄较大与 OS 恶化相关。多因素分析显示，FIGO 分期与 DFS（HR 1.98；95%CI 1.05-3.71）和 OS（HR 1.76；95%CI 1.00-3.10）均显著相关，而年龄影响 OS 率（HR 1.78；95%CI 1.04-2.95）。
mAgNOR 和 pAgNOR 是细胞动力学的有用标志物。需要在更大的人群中进行前瞻性研究，以确认这些结果在 AgNORs 对生存的影响方面的意义。
