Xie X, Stenersen T C, Clausen O P, Boysen M
Department of Otolaryngology, National Hospital, University of Oslo, Norway.
Head Neck. 1997 Jan;19(1):20-6. doi: 10.1002/(sici)1097-0347(199701)19:1<20::aid-hed4>3.0.co;2-x.
The quantity of nucleolar organizer regions (AgNORs) appear to be prognostic significant in several tumor types.
Sections from 93 routinely processed pretreatment biopsies from patients with glottic carcinomas were stained by silver nitrate and evaluated by two counting methods: (1) the mean number of AgNOR per tumor nucleus (mAgNOR), and (2) the number of tumor nuclei with one, two, three, four, and more than four AgNOR grains. From these figures the percentage of nuclei with one, two or less, three or less, and four or less AgNORs (pAgNOR1, aAgNOR < or = 2 etc) were calculated.
The median mAgNOR was 4.3, and low counts correlated favorably with the disease-free period (p = 0.0433). The median percentages for pAgNORs were 14, 26, 38, and 51 for pAgNor1, PAgNOR < or = 2, pAgNor < or = 3 and pAgNOR, < or = 4 respectively. Values above the medians correlated positively with the disease-free period (p-values ranging from 0.0005 to 0.0001). Although pAgNOR < or = 3 appeared to be the best discriminator by multivariate analysis, pAgNOR1 is the method of choice because this parameter is the easiest and quickest to perform.
pAgNOR counts appear to be a potent prognostic marker and may become useful in treatment decisions.
核仁组成区（AgNORs）的数量在几种肿瘤类型中似乎具有预后意义。
对93例声门癌患者预处理活检的常规切片进行硝酸银染色，并通过两种计数方法进行评估：（1）每个肿瘤细胞核的AgNOR平均数量（mAgNOR），以及（2）具有1个、2个、3个、4个及4个以上AgNOR颗粒的肿瘤细胞核数量。根据这些数据计算出具有1个、2个及以下、3个及以下和4个及以下AgNORs的细胞核百分比（pAgNOR1、pAgNOR≤2等）。
mAgNOR的中位数为4.3，低计数与无病生存期呈良好相关性（p = 0.0433）。pAgNORs的中位数百分比分别为：pAgNor1为14%，pAgNOR≤2为26%，pAgNor≤3为38%，pAgNOR≤4为51%。高于中位数的值与无病生存期呈正相关（p值范围为0.0005至0.0001）。虽然通过多变量分析pAgNOR≤3似乎是最佳判别指标，但pAgNOR1是首选方法，因为该参数执行起来最简单、最快。
pAgNOR计数似乎是一种有效的预后标志物，可能有助于治疗决策。
