Rogalska Aneta, Forma Ewa, Ciesielski Piotr, Bryś Magdalena, Krześlak Anna, Marczak Agnieszka
Department of Thermobiology, Faculty of Biology and Environmental Protection, University of Lodz, Poland.
Department of Cytobiochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Poland.
Prz Menopauzalny. 2014 Jun;13(3):155-61. doi: 10.5114/pm.2014.43817. Epub 2014 Jun 29.
Ovarian cancer is one of the most difficult problems in gynecologic oncology and the search for new drugs effective in the treatment of this kind of cancer, especially in cases resistant to current forms of therapy, remains a challenging priority.
The aim of the study was to analyze the effect of metformin on apoptosis and the BIRC5 gene expression in ovarian cancer cell line SKOV-3. The BIRC5 gene encodes survivin protein.
SKOV-3 cells were treated with metformin (10 mM). Apoptotic changes in studied cells were analyzed by double staining using a mixture of fluorochromes - Hoechst 33258/propidium iodide (PI). The expression of the BIRC5 gene at the mRNA level was analyzed using the real-time PCR technique.
Treatment of cells with metformin causes changes in the cell shape from oval to spindle and leads to the separation of the cells from the monolayer. Furthermore, metformin induces apoptosis and necrosis of ovarian cancer cells. A statistically significant increase in the number of apoptotic cells after 48 and 72 hours' treatment with metformin relative to a control cells seems to be correlated with a decrease in the expression of the BIRC5 gene at the mRNA level.
Metformin seems to be a promising agent, whose use in ovarian cancer patients may contribute to improving the effectiveness of therapy.
卵巢癌是妇科肿瘤学中最棘手的问题之一，寻找对这种癌症有效的新药，尤其是对当前治疗形式耐药的病例，仍然是一项具有挑战性的首要任务。
本研究旨在分析二甲双胍对卵巢癌细胞系SKOV-3凋亡及BIRC5基因表达的影响。BIRC5基因编码生存素蛋白。
用二甲双胍（10 mM）处理SKOV-3细胞。通过使用荧光染料混合物Hoechst 33258/碘化丙啶（PI）进行双重染色分析所研究细胞中的凋亡变化。使用实时PCR技术分析BIRC5基因在mRNA水平的表达。
用二甲双胍处理细胞会导致细胞形状从椭圆形变为纺锤形，并导致细胞与单层分离。此外，二甲双胍可诱导卵巢癌细胞凋亡和坏死。与对照细胞相比，用二甲双胍处理48小时和72小时后凋亡细胞数量的统计学显著增加似乎与mRNA水平上BIRC5基因表达的降低相关。
二甲双胍似乎是一种有前景的药物，其在卵巢癌患者中的应用可能有助于提高治疗效果。
