Simonsson M, Eriksson S, Håkanson R, Lind T, Lönroth H, Lundell L, O'Connor D T, Sundler F
Dept. of Medical Cell Research, University of Lund, Sweden.
Scand J Gastroenterol. 1988 Nov;23(9):1089-99. doi: 10.3109/00365528809090174.
The oxyntic mucosa of the human stomach harbors at least five different endocrine cell types (ECL cells, A-like or X cells, somatostatin cells (D), enterochromaffin (EC) cells, and D1 or P cells). Little is known about their functional roles, and of the hormones they produce only somatostatin has been identified. The relative frequency and regional distribution of the different endocrine cell populations were studied in 13 adults with no manifest gastrointestinal disease. From each of them at least three biopsy specimens were taken at seven fixed locations within the oxyntic mucosa. The specimens were examined for the different endocrine cell types by means of immunocytochemistry (staining with antisera against chromogranin A,5-hydroxytryptamine, and somatostatin) and silver staining techniques (demonstration of argyrophil cells by the methods of Grimelius or Sevier-Munger). Chromogranin-positive cells included all endocrine cells identified by the other staining techniques. Grimelius-positive cells included all endocrine cells except the somatostatin cells. Sevier-Munger-positive cells, finally, included the ECL cells and the EC cells. The frequency of ECL cells could be calculated by subtracting the number of EC cells from the number of Sevier-Munger-positive cells. The ECL cells represented 35% of the total endocrine number, somatostatin cells 26%, and EC cells 25%. The remaining 14% consisted of A-like cells, D1 cells, and P cells. Generally, the endocrine cells predominated in the basal portion of the glands, but the various populations of endocrine cells were not uniformly distributed in the various regions of the oxyntic mucosa. However, representative specimens could be obtained from the main body of the stomach, and the results indicate that the examination of a fairly small number of specimens from the main body of the stomach may be sufficient for assessing the frequency of endocrine cells in the oxyntic mucosa of individual patients.
人类胃的泌酸黏膜至少包含五种不同类型的内分泌细胞（肠嗜铬样细胞、A 样或 X 细胞、生长抑素细胞（D 细胞）、肠嗜铬细胞（EC 细胞）以及 D1 或 P 细胞）。人们对它们的功能作用知之甚少，而且它们所产生的激素中，仅生长抑素已被识别。在 13 名无明显胃肠道疾病的成年人中，研究了不同内分泌细胞群体的相对频率和区域分布。从他们每个人的泌酸黏膜内七个固定位置至少采集三个活检标本。通过免疫细胞化学（用抗嗜铬粒蛋白 A、5 - 羟色胺和生长抑素的抗血清染色）和银染色技术（用 Grimelius 法或 Sevier - Munger 法显示嗜银细胞）检查标本中的不同内分泌细胞类型。嗜铬粒蛋白阳性细胞包括通过其他染色技术识别的所有内分泌细胞。Grimelius 阳性细胞包括除生长抑素细胞外的所有内分泌细胞。最后，Sevier - Munger 阳性细胞包括肠嗜铬样细胞和肠嗜铬细胞。肠嗜铬样细胞的频率可通过从 Sevier - Munger 阳性细胞数量中减去肠嗜铬细胞数量来计算。肠嗜铬样细胞占内分泌细胞总数的 35%，生长抑素细胞占 26%，肠嗜铬细胞占 25%。其余 14%由 A 样细胞、D1 细胞和 P 细胞组成。一般来说，内分泌细胞在腺体基部占主导，但不同类型的内分泌细胞群体在泌酸黏膜的不同区域分布并不均匀。然而，可从胃体获取代表性标本，结果表明，检查来自胃体的相当少量标本可能足以评估个体患者泌酸黏膜内分泌细胞的频率。
