Wiersma E J, Coulie P G, Heyman B
Department of Immunology, Uppsala University, Sweden.
Scand J Immunol. 1989 Apr;29(4):439-48. doi: 10.1111/j.1365-3083.1989.tb01143.x.
Nine monoclonal IgG-anti-TNP antibodies were investigated for their ability to modulate anticarrier responses in mice immunized with sheep red blood cells-2,4,6-trinitrophenyl (SRBC-TNP) or keyhole limpet haemocyanin-TNP (KLH-TNP). The antibodies enhanced the anticarrier response when KLH-TNP was used as antigen but suppressed it when SRBC-TNP was used. The enhancing and suppressive effects were not exerted by entirely the same sets of antibodies. The suppression was correlated to efficient antigen binding, but not complement activation, haemagglutination, or isotype of the monoclonal antibodies. In contrast, enhancement was correlated to isotype and complement activation but not to antigen binding capacity. Both the enhancing and the suppressive effects seem to require Fc-mediated functions of the IgG molecules since they modulate the anti-carrier response although they recognize hapten determinants. Thus, one and the same monoclonal hapten-specific IgG-antibody can enhance the anti-KLH response up to 38-fold whereas it suppresses the anti-SRBC response by more that 10-fold.
研究了九种单克隆IgG抗三硝基苯（TNP）抗体调节用绵羊红细胞-2,4,6-三硝基苯（SRBC-TNP）或钥孔戚血蓝蛋白-TNP（KLH-TNP）免疫的小鼠中抗载体反应的能力。当使用KLH-TNP作为抗原时，这些抗体增强了抗载体反应，但当使用SRBC-TNP时则抑制了该反应。增强和抑制作用并非完全由同一组抗体发挥。抑制作用与有效的抗原结合相关，但与补体激活、血凝或单克隆抗体的同种型无关。相反，增强作用与同种型和补体激活相关，但与抗原结合能力无关。增强和抑制作用似乎都需要IgG分子的Fc介导功能，因为它们虽然识别半抗原决定簇，但却调节抗载体反应。因此，同一种单克隆半抗原特异性IgG抗体可将抗KLH反应增强多达38倍，而将抗SRBC反应抑制超过10倍。
