Anastassova-Kristeva M, Kancheva L, Martinova Y, Toshkov A, Todorov T
Zentralbl Bakteriol Orig A. 1978 Nov;242(1):63-70.
L-forms of Erysipelotrix rhusiopathiae labelled with 3H-thymidine were intravenously administered to albino mice. Autoradiographic studies of the liver, kidney and spleen were undertaken at periods ranging from 2 minutes to 15 days. On the second minute following the administration of the radioactive material whole labelled microorganisms and chains of silver grains were recovered in the examined organs. Up to the 15th minute labels were observed also in the cells of the RES. Following the 30th minute the silver grains were positioned at a characteristic site in the Golgi region of the hepatocytes. At the same time in the kidney they were localized in the glomerular space and in the lumen of the renal tubules, whereas in the spleen - mainly around the megakaryocytes. By the 15th day labelling gradually diminished, single silver grains being found over some nuclei of megakaryocytes, liver and kidney parenchymal cells. The present study throws light over some aspects of the interrelationship between the micro- and macroorganism concerning the mechanisms of desintegration, elimination and the uptaking of labelled microbial DNA.
将用³H - 胸腺嘧啶核苷标记的猪丹毒杆菌L型静脉注射给白化小鼠。在2分钟至15天的时间段内，对肝脏、肾脏和脾脏进行放射自显影研究。在给予放射性物质后的第2分钟，在所检查的器官中发现了完整的标记微生物和银粒链。直至第15分钟，在网状内皮系统（RES）的细胞中也观察到标记物。在第30分钟之后，银粒位于肝细胞高尔基体区域的特定部位。与此同时，在肾脏中它们定位于肾小球间隙和肾小管腔内，而在脾脏中 - 主要围绕巨核细胞。到第15天时，标记逐渐减少，在巨核细胞、肝脏和肾脏实质细胞的一些细胞核上发现单个银粒。本研究揭示了微生物与宏观生物体之间在 disintegration、消除和摄取标记微生物DNA机制方面相互关系的一些方面。 （注：原文中“desintegration”可能有误，推测应为“ disintegration”，意为解体、崩解 ）
