Rukasz H
Wiad Parazytol. 1989;35(1):19-24.
The material consisted of 20 male guinea pigs of body weight 300 +/- 50 g. Fifteen of them were infected per os with a dose of 5000 invasive eggs of A. suum. At the 4th, 7th and 20th days of infection the animals were dissected and sections of kidney were taken for histopathological and histochemical studies. The sections were stained with hematoxylin and eosin. The presence of mucopolisachcarides was detected with the paS method, the activity of acid and alkaline phosphatases--with Gomori's method. At the 4th and 7th day of infection an active congestion of the kidney, dilation of blood vessels and changes in the size of epithelial cells of proximal concoluted tubules were observed. The epithelium of the tubules was high, often closing their lumen. Moreover, at the 7th day of infection small ecchymosis was noted. On the sections stained with the paS method a distinct thickening of the basement membrane of the parietal lamellat of Bowman sac was found at the 4th and 7th day of infection, as well as a weaker staining of brush border in main parts of nephrons. A tightening of the vasculary loop of some glomeruli was also noted at the 7th day. At the 20th day of the experiment changes were observed only in single glomeruli in the vasculary loop. At the 4th day of infection an increase in the activity of the acid and alkaline phosphatases was observed in the kidney, followed by a decrease in the activity of both these enzymes at the 7th and 20th day.
材料包括20只体重为300±50克的雄性豚鼠。其中15只经口感染5000个侵袭性猪蛔虫卵。在感染后的第4天、第7天和第20天，解剖动物并取肾脏切片进行组织病理学和组织化学研究。切片用苏木精和伊红染色。用PAS法检测粘多糖的存在，用Gomori法检测酸性和碱性磷酸酶的活性。在感染后的第4天和第7天，观察到肾脏有活动性充血、血管扩张以及近端曲管上皮细胞大小的变化。肾小管上皮细胞高，常使管腔闭合。此外，在感染后的第7天发现有小的瘀斑。在用PAS法染色的切片上，在感染后的第4天和第7天，发现鲍曼囊壁层的基底膜明显增厚，以及肾单位主要部分刷状缘的染色变浅。在第7天还注意到一些肾小球的血管袢变紧。在实验的第20天，仅在血管袢的单个肾小球中观察到变化。在感染后的第4天，观察到肾脏中酸性和碱性磷酸酶的活性增加，随后在第7天和第20天这两种酶的活性均下降。
