Liquid holding recovery in yeast rad3 mutant after treatment with diepoxybutane: effect on mutations, recombinations and molecular weight of DNA.

It was found that, in cells of the rad3 mutant treated with diepoxybutane (DEB), two systems of repair operate during the liquid holding period. One of them is independent of "de novo" protein synthesis and leads to increased cell survival. The other is dependent on protein synthesis, affects the frequency of mitotic recombinations and mutations and is responsible for changes in molecular weight of the damaged DNA.