Tohyama K, Ohmori S, Michishita M, Ueda T, Ueda Y, Sakoda H, Yoshida Y, Uchino H
First Division of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.
Eur J Haematol. 1989 Apr;42(4):348-53. doi: 10.1111/j.1600-0609.1989.tb01224.x.
To evaluate the effects of recombinant G-CSF and GM-CSF on RAEB and RAEB-T cells, blast cells from 6 patients were incubated in liquid culture systems with these CSFs for 7 days, and their numerical, morphological and functional changes were assessed. Both CSFs stimulated cell growth, but decreased the proportion of blast cells in 5 of the 6 cases. Karyotypic abnormalities persisted during cultivation in some cases. The CSFs also stimulated the expression of part of the esterase activities, and a positive interaction of both CSFs was seen in part. Although CSFs had no significant effects on the ability of cells to reduce NBT or to phagocytize latex particles, the results indicated that they induce partial differentiation of blast cells. It appears that such pathological cells still retain the capacity to respond to growth factors.
为评估重组人粒细胞集落刺激因子（G-CSF）和粒细胞-巨噬细胞集落刺激因子（GM-CSF）对难治性贫血伴原始细胞过多（RAEB）和转化中的难治性贫血伴原始细胞过多（RAEB-T）细胞的作用，将6例患者的原始细胞在液体培养系统中与这些集落刺激因子一起培养7天，并评估其数量、形态和功能变化。两种集落刺激因子均刺激细胞生长，但6例中有5例原始细胞比例下降。在某些情况下，核型异常在培养过程中持续存在。集落刺激因子还刺激了部分酯酶活性的表达，并且部分观察到两种集落刺激因子的正性相互作用。尽管集落刺激因子对细胞还原硝基蓝四唑（NBT）或吞噬乳胶颗粒的能力没有显著影响，但结果表明它们可诱导原始细胞部分分化。看来这种病理性细胞仍保留对生长因子作出反应的能力。
