Tohyama K, Ohmori S, Ueda T, Ueda Y, Sakoda H, Yoshida Y, Uchino H
First Division of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.
Blut. 1989 Apr;58(4):181-6. doi: 10.1007/BF00320770.
We added recombinant human gamma-interferon (gamma-IFN) and 1 alpha, 25-dihydroxyvitamin D3 (1 alpha, 25 (OH)2D3) to the bone marrow cells from six patients with RAEB or RAEB-T in liquid suspension cultures. After cultivation for 7 to 9 days, numerical, morphological and functional changes of the cells were assessed. gamma-IFN and 1 alpha, 25 (OH)2D3 additively suppressed cell growth, especially the number of blast cells decreased. The expression of alpha-naphthylbutyrate esterase (NBE) activity appeared to be promoted but that of naphthol AS-D chloroacetate esterase (NAE) activity was apparently suppressed by the addition of gamma-IFN and/or 1 alpha, 25 (OH)2D3. The percentage of NBT reduction-positive cells and latex-phagocytizing cells was only slightly increased by both agents. These results indicate that gamma-IFN and 1 alpha, 25 (OH)2D3 cooperate to induce monocytoid differentiation of the patients' blast cells. Combination therapy with both agents merits further study.
我们将重组人γ干扰素（γ-IFN）和1α，25-二羟维生素D3（1α，25（OH）2D3）添加到6例难治性贫血伴原始细胞增多（RAEB）或难治性贫血伴原始细胞增多转变型（RAEB-T）患者的骨髓细胞悬液培养体系中。培养7至9天后，评估细胞的数量、形态和功能变化。γ-IFN和1α，25（OH）2D3协同抑制细胞生长，尤其是原始细胞数量减少。添加γ-IFN和/或1α，25（OH）2D3后，α-萘丁酸酯酶（NBE）活性的表达似乎增强，而萘酚AS-D氯乙酸酯酶（NAE）活性明显受到抑制。两种药物仅使硝基蓝四氮唑（NBT）还原阳性细胞和乳胶吞噬细胞的百分比略有增加。这些结果表明，γ-IFN和1α，25（OH）2D3协同诱导患者原始细胞向单核细胞样分化。两种药物的联合治疗值得进一步研究。
