Nagineni C N, Bhat S P
Jules Stein Eye Institute, University of California, School of Medicine, Los Angeles 90024-1771.
FEBS Lett. 1989 May 22;249(1):89-94. doi: 10.1016/0014-5793(89)80022-5.
We have recently shown the presence of alpha B-crystallin in non-ocular tissues of diverse embryological origins such as the heart, brain, spinal cord, kidney, retina, etc. Using an alpha B-crystallin-specific antiserum and immunofluorescence, immunoblotting, immunoprecipitation and peptide mapping with Staphylococcus aureus protease, we demonstrate differential expression of alpha B-crystallin in epithelial and fibroblast cell lines. alpha B-Crystallin was detectable only in epithelial cell lines such as MDBK, MDCK, LLCPK1 and JTC-12, and was not observed in two kidney fibroblast cell lines, one skin fibroblast cell line, and one corneal fibroblast cell line. Differential expression of the alpha B-crystallin gene was also confirmed by Northern blot analysis of the RNAs isolated from these cell lines. These data suggest a cell-type-specific role for alpha B.
我们最近发现，αB-晶状体蛋白存在于多种胚胎起源的非眼部组织中，如心脏、大脑、脊髓、肾脏、视网膜等。利用αB-晶状体蛋白特异性抗血清以及免疫荧光、免疫印迹、免疫沉淀和金黄色葡萄球菌蛋白酶肽图谱分析，我们证明了αB-晶状体蛋白在上皮细胞系和成纤维细胞系中的差异表达。仅在诸如MDBK、MDCK、LLCPK1和JTC-12等上皮细胞系中可检测到αB-晶状体蛋白，而在两个肾成纤维细胞系、一个皮肤成纤维细胞系和一个角膜成纤维细胞系中未观察到。从这些细胞系中分离的RNA的Northern印迹分析也证实了αB-晶状体蛋白基因的差异表达。这些数据表明αB具有细胞类型特异性作用。
