在果蝇黑腹果蝇乙醇脱氢酶基因（adh）5'端插入一个copia元件，与发育和组织特异性表达模式的改变有关。
Insertion of a copia element 5' to the Drosophila melanogaster alcohol dehydrogenase gene (adh) is associated with altered developmental and tissue-specific patterns of expression.
作者信息
Strand D J, McDonald J F
机构信息
Department of Genetics, University of Georgia, Athens 30602.
出版信息
Genetics. 1989 Apr;121(4):787-94. doi: 10.1093/genetics/121.4.787.
Abstract
The Drosophila melanogaster alcohol dehydrogenase gene (adh) is under the control of two separate promoters (proximal and distal) which are preferentially utilized at the larval and adult life stages, respectively. A variant alcohol dehydrogenase allele (RI-42) isolated from a natural population contains a copia retroviral-like transposable element inserted 240 bp upstream from the distal (adult) adh transcriptional start site. Levels of adh transcripts in the RI-42 variant are reduced in tissues and at life stages where copia is actively expressed and are affected in trans- by mutant alleles at the suppressor-of-white-apricot (su(wa] and suppressor-of-forked (su(f] loci. These suppressor genes have no effect on adh expression in wild-type Drosophila.
摘要
黑腹果蝇乙醇脱氢酶基因（adh）受两个独立启动子（近端和远端）的调控，它们分别在幼虫和成虫生命阶段被优先利用。从自然种群中分离出的一个乙醇脱氢酶变异等位基因（RI - 42），在远端（成虫）adh转录起始位点上游240 bp处插入了一个类反转录病毒转座因子copia。在copia活跃表达的组织和生命阶段，RI - 42变异体中adh转录本的水平降低，并且受到白色杏色抑制基因（su(wa)）和叉状抑制基因（su(f)）位点的突变等位基因的反式影响。这些抑制基因对野生型果蝇中的adh表达没有影响。
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本文引用的文献
1
Biochemical differences between products of the Adh locus in Drosophila.果蝇中乙醇脱氢酶（Adh）基因座产物之间的生化差异。
Genetics. 1980 Aug;95(4):1013-22. doi: 10.1093/genetics/95.4.1013.
2
Isolation and partial characterization of the Drosophila alcohol dehydrogenase gene.果蝇乙醇脱氢酶基因的分离与部分特性分析
Proc Natl Acad Sci U S A. 1980 Oct;77(10):5794-8. doi: 10.1073/pnas.77.10.5794.
3
Biochemical and molecular analysis of naturally occurring Adh variants in Drosophila melanogaster.黑腹果蝇中天然存在的乙醇脱氢酶变体的生化与分子分析
Proc Natl Acad Sci U S A. 1983 Aug;80(15):4798-802. doi: 10.1073/pnas.80.15.4798.
4
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.一种将DNA限制性内切酶片段放射性标记至高比活度的技术。
Anal Biochem. 1983 Jul 1;132(1):6-13. doi: 10.1016/0003-2697(83)90418-9.
5
Drosophila melanogaster mutations suppressible by the suppressor of Hairy-wing are insertions of a 7.3-kilobase mobile element.可被毛翅抑制子抑制的黑腹果蝇突变是一个7.3千碱基移动元件的插入。
Proc Natl Acad Sci U S A. 1983 Mar;80(6):1678-82. doi: 10.1073/pnas.80.6.1678.
6
Cloning of DNA sequences from the white locus of D. melanogaster by a novel and general method.通过一种新颖通用的方法克隆黑腹果蝇白色基因座的DNA序列。
Cell. 1981 Sep;25(3):693-704. doi: 10.1016/0092-8674(81)90176-8.
7
Copia-like transposable elements in the Drosophila genome.果蝇基因组中的类Copia转座元件。
Cold Spring Harb Symp Quant Biol. 1981;45 Pt 2:619-28. doi: 10.1101/sqb.1981.045.01.080.
8
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EMBO J. 1984 Nov;3(11):2543-8. doi: 10.1002/j.1460-2075.1984.tb02170.x.
9
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10
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