Bartkiewicz M, Gold H, Altman S
Department of Biology, Yale University, New Haven, Connecticut 06520.
Genes Dev. 1989 Apr;3(4):488-99. doi: 10.1101/gad.3.4.488.
An RNA molecule, 340 nucleotides in length and designated H1 RNA, copurifies with RNase P activity from extracts of HeLa cells or isolated HeLa cell nuclei. When the genomic DNA of various organisms is probed with H1 cDNA in Southern hybridization assays, only mammalian DNA gives a positive signal. The gene coding for H1 RNA in human cells is present in one to three copies per cell. The nucleotide sequence of H1 RNA, which shows little homology to the known sequences of its analogs from prokaryotes and yeast, can be drawn as a two-dimensional, hydrogen-bonded structure that resembles similar structures proposed for the RNA subunit of RNase P from these other sources. Part of the hypothetical structure is virtually identical to structures that can be drawn for analogous RNAs from Saccharomyces cerevisiae, Schizosaccharomyces pombe, and S. octosporus.
一种长度为340个核苷酸、名为H1 RNA的RNA分子，可与来自HeLa细胞提取物或分离出的HeLa细胞核的RNase P活性共同纯化。当在Southern杂交试验中用H1 cDNA探测各种生物体的基因组DNA时，只有哺乳动物DNA给出阳性信号。人类细胞中编码H1 RNA的基因每个细胞中有1至3个拷贝。H1 RNA的核苷酸序列与来自原核生物和酵母的类似物的已知序列几乎没有同源性，它可以绘制成一种二维氢键结构，类似于从这些其他来源提出的RNase P的RNA亚基的类似结构。假设结构的一部分实际上与酿酒酵母、粟酒裂殖酵母和八孢裂殖酵母的类似RNA可以绘制的结构相同。
