Tasaka K, Mio M
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Agents Actions. 1989 Apr;27(1-2):79-82. doi: 10.1007/BF02222204.
When sensitized guinea-pig lung mast cells were exposed to antigen, granules were pushed out on to the cell surface. Subsequently, thin filaments, some extending as long as 15 microns, were projected radially with the extruded granules. The latter became swollen in the extracellular medium and the elongated filaments became shorter, until, within 7-8 min, the granules were reincorporated into the cytoplasm. The time course of morphological changes corresponded approximately to that of changes in the intracellular Ca2+ concentrations. The filaments connecting the extruded granules to the cell surface were stained with rhodamine-phalloidin, indicating that they consisted mainly of actin.
当致敏豚鼠肺肥大细胞暴露于抗原时，颗粒被推出到细胞表面。随后，一些长达15微米的细丝随着挤出的颗粒呈放射状伸出。后者在细胞外介质中肿胀，细长的细丝变短，直到在7-8分钟内，颗粒重新并入细胞质中。形态变化的时间进程大致与细胞内Ca2+浓度的变化时间进程一致。将挤出的颗粒连接到细胞表面的细丝用罗丹明-鬼笔环肽染色，表明它们主要由肌动蛋白组成。
