Psenicnik M, Pipan N
Institute of Human Biology, Medical Faculty, Ljubljana, Yugoslavia.
Histochemistry. 1989;91(5):445-7. doi: 10.1007/BF00493833.
Osmium impregnation was used upon the mice stomach epithelium to show possible differences in staining during differentiation. In the cells of the stratified gastric epithelium of 14-day-old mice embryos Os black was completely lacking in the Golgi complex. In some but not all cells the staining appears in the perinuclear space and in the endoplasmic reticulum. In the mucoid cells 1 and 8 days after the birth the osmiophility is not uniformly distributed throughout the endomembrane segments, except the cis face of the Golgi complex which is heavily stained. Our results indicate on the variability of the reduction capacity of particular endomembrane segments during differentiation and among the cells at the definite developmental stage.
采用锇浸染法处理小鼠胃上皮,以显示分化过程中染色可能存在的差异。在14日龄小鼠胚胎的复层胃上皮细胞中,锇黑在高尔基体复合体中完全缺失。在部分而非所有细胞中,染色出现在核周间隙和内质网中。在出生后1天和8天的黏液细胞中,嗜锇性在整个内膜段分布不均,除了高尔基体复合体的顺面被重度染色。我们的结果表明,在分化过程中以及特定发育阶段的细胞之间,特定内膜段的还原能力存在变异性。