Samuel L H, Flickinger C J
Cell Tissue Res. 1987 Jan;247(1):203-13. doi: 10.1007/BF00216563.
Two male sex accessory glands of the mouse, seminal vesicle and coagulating gland, were compared with the aim of relating differences in the morphology of organelles to the kinetics of the secretory process. The epithelial cells of the two glands were assessed by morphometric analysis, cytochemical staining, and electron-microscopic autoradiography after administration of a labeled amino acid. The rough endoplasmic reticulum of the seminal vesicle comprised narrow parallel cisternae, while that of the coagulating gland was greatly distended and occupied a much larger percentage of the cytoplasmic volume. Radioactively labeled products were secreted much more rapidly in the seminal vesicle than in the coagulating gland. The primary point of difference in kinetics of intracellular transport between the two glands was in exit of material from the rough endoplasmic reticulum. The more rapid drainage of the rough endoplasmic reticulum may be related to its relatively greater membrane surface density and lesser internal volume. In contrast, similarities in size and cytochemical staining in the Golgi apparatus of the two glands were accompanied by similar kinetics of intracellular transport of secretory protein through this organelle.
为了将细胞器形态学上的差异与分泌过程的动力学联系起来，对小鼠的两个雄性附属性腺——精囊和凝固腺进行了比较。在给予标记氨基酸后，通过形态计量分析、细胞化学染色和电子显微镜放射自显影对这两个腺体的上皮细胞进行了评估。精囊的粗面内质网由狭窄的平行池组成，而凝固腺的粗面内质网则明显扩张，占据细胞质体积的比例更大。放射性标记产物在精囊中的分泌速度比在凝固腺中快得多。这两个腺体在细胞内运输动力学上的主要差异点在于物质从粗面内质网的排出。粗面内质网更快的排空可能与其相对更大的膜表面密度和更小的内部体积有关。相比之下，两个腺体高尔基体在大小和细胞化学染色上的相似性伴随着分泌蛋白通过该细胞器的细胞内运输动力学的相似性。
