Winborn W B, Seelig L L
J Cell Biol. 1974 Oct;63(1):99-108. doi: 10.1083/jcb.63.1.99.
Parietal cells of the stomach of the hamster show extensive amounts of dense material in a variety of organelles after prolonged exposure to a solution of osmium tetroxide. Conspicuous amounts of reduced osmium compounds are evident within the granular endoplasmic reticulum, perinuclear cisterna, and vesicular elements of the Golgi complex. Dense material is also apparent within cristae of the mitochondria, the surface coat of the microvilli of the intracellular canaliculus, and vesicular elements of the multivesicular bodies. Multivesicular bodies, containing numerous small osmiophilic elements, are often seen surrounding and/or in close contact with mitochondria. The proximity of the multivesicular bodies to the mitochondria appears to be related to an autophagic process involving degradation of mitochondria. The distribution and intensity of the precipitates within the organelles of the parietal cells vary in different regions of the gastric glands. The findings of this study emphasize that cell structures other than the Golgi complex may contain large concentrations of reduced osmium compounds after prolonged exposure to a solution of osmium tetroxide.
仓鼠胃的壁细胞在长时间暴露于四氧化锇溶液后，其各种细胞器中显示出大量致密物质。在颗粒内质网、核周池和高尔基体复合体的囊泡成分中，明显可见大量还原态锇化合物。线粒体嵴内、细胞内小管微绒毛的表面涂层以及多囊泡体的囊泡成分中也可见致密物质。含有众多小嗜锇性成分的多囊泡体常可见于线粒体周围和/或与其紧密接触。多囊泡体与线粒体的接近似乎与涉及线粒体降解的自噬过程有关。壁细胞细胞器内沉淀物的分布和强度在胃腺的不同区域有所不同。本研究结果强调，除高尔基体复合体外，长时间暴露于四氧化锇溶液后，其他细胞结构可能含有高浓度的还原态锇化合物。
