• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

环状三链螺旋形成寡核苷酸RNA对猫传染性腹膜炎病毒复制的体外抗病毒活性

In vitro antiviral activity of circular triple helix forming oligonucleotide RNA towards Feline Infectious Peritonitis virus replication.

作者信息

Choong Oi Kuan, Mehrbod Parvaneh, Tejo Bimo Ario, Omar Abdul Rahman

机构信息

Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.

Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia ; Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.

出版信息

Biomed Res Int. 2014;2014:654712. doi: 10.1155/2014/654712. Epub 2014 Feb 20.

DOI:10.1155/2014/654712
PMID:24707494
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3950953/
Abstract

Feline Infectious Peritonitis (FIP) is a severe fatal immune-augmented disease in cat population. It is caused by FIP virus (FIPV), a virulent mutant strain of Feline Enteric Coronavirus (FECV). Current treatments and prophylactics are not effective. The in vitro antiviral properties of five circular Triple-Helix Forming Oligonucleotide (TFO) RNAs (TFO1 to TFO5), which target the different regions of virulent feline coronavirus (FCoV) strain FIPV WSU 79-1146 genome, were tested in FIPV-infected Crandell-Rees Feline Kidney (CRFK) cells. RT-qPCR results showed that the circular TFO RNAs, except TFO2, inhibit FIPV replication, where the viral genome copy numbers decreased significantly by 5-fold log10 from 10(14) in the virus-inoculated cells to 10(9) in the circular TFO RNAs-transfected cells. Furthermore, the binding of the circular TFO RNA with the targeted viral genome segment was also confirmed using electrophoretic mobility shift assay. The strength of binding kinetics between the TFO RNAs and their target regions was demonstrated by NanoITC assay. In conclusion, the circular TFOs have the potential to be further developed as antiviral agents against FIPV infection.

摘要

猫传染性腹膜炎（FIP）是猫群体中一种严重的致命性免疫增强疾病。它由猫肠道冠状病毒（FECV）的一种毒性突变株——猫传染性腹膜炎病毒（FIPV）引起。目前的治疗方法和预防措施均无效。在感染FIPV的克兰德尔 - 里斯猫肾（CRFK）细胞中测试了五种靶向毒性猫冠状病毒（FCoV）毒株FIPV WSU 79 - 1146基因组不同区域的环状三链螺旋形成寡核苷酸（TFO）RNA（TFO1至TFO5）的体外抗病毒特性。逆转录定量聚合酶链反应（RT - qPCR）结果表明，除TFO2外，环状TFO RNA可抑制FIPV复制，病毒基因组拷贝数从接种病毒的细胞中的10（14）显著下降5个对数10倍，至环状TFO RNA转染细胞中的10（9）。此外，还使用电泳迁移率变动分析证实了环状TFO RNA与靶向病毒基因组片段的结合。纳米等温滴定量热法（NanoITC）分析证明了TFO RNA与其靶区域之间结合动力学的强度。总之，环状TFO有潜力进一步开发成为抗FIPV感染的抗病毒药物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e46d/3950953/0ce3bf9a8017/BMRI2014-654712.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e46d/3950953/d6cc2b6f7be7/BMRI2014-654712.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e46d/3950953/505fefadafde/BMRI2014-654712.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e46d/3950953/c57b420828f1/BMRI2014-654712.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e46d/3950953/0ce3bf9a8017/BMRI2014-654712.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e46d/3950953/d6cc2b6f7be7/BMRI2014-654712.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e46d/3950953/505fefadafde/BMRI2014-654712.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e46d/3950953/c57b420828f1/BMRI2014-654712.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e46d/3950953/0ce3bf9a8017/BMRI2014-654712.004.jpg

相似文献

1
In vitro antiviral activity of circular triple helix forming oligonucleotide RNA towards Feline Infectious Peritonitis virus replication.环状三链螺旋形成寡核苷酸RNA对猫传染性腹膜炎病毒复制的体外抗病毒活性
Biomed Res Int. 2014;2014:654712. doi: 10.1155/2014/654712. Epub 2014 Feb 20.
2
Establishment of Full-Length cDNA Clones and an Efficient Oral Infection Model for Feline Coronavirus in Cats.建立全长 cDNA 克隆及猫冠状病毒在猫中的高效口服感染模型。
J Virol. 2021 Oct 13;95(21):e0074521. doi: 10.1128/JVI.00745-21. Epub 2021 Aug 18.
3
In vitro inhibition of feline coronavirus replication by small interfering RNAs.小干扰 RNA 对猫冠状病毒复制的体外抑制作用。
Vet Microbiol. 2011 Jun 2;150(3-4):220-9. doi: 10.1016/j.vetmic.2011.01.023. Epub 2011 Feb 1.
4
Establishment of a Virulent Full-Length cDNA Clone for Type I Feline Coronavirus Strain C3663.建立一株 I 型猫冠状病毒 C3663 的强毒全长 cDNA 克隆。
J Virol. 2019 Oct 15;93(21). doi: 10.1128/JVI.01208-19. Print 2019 Nov 1.
5
Mutation analysis of the spike protein in Italian feline infectious peritonitis virus and feline enteric coronavirus sequences.对意大利猫传染性腹膜炎病毒和猫肠道冠状病毒序列的刺突蛋白进行突变分析。
Res Vet Sci. 2021 Mar;135:15-19. doi: 10.1016/j.rvsc.2020.12.023. Epub 2021 Jan 2.
6
Transcriptional profiling of feline infectious peritonitis virus infection in CRFK cells and in PBMCs from FIP diagnosed cats.猫传染性腹膜炎病毒感染 CRFK 细胞和 FIP 确诊猫 PBMCs 的转录组分析。
Virol J. 2013 Nov 9;10:329. doi: 10.1186/1743-422X-10-329.
7
Sensitivity and specificity of a real-time reverse transcriptase polymerase chain reaction detecting feline coronavirus mutations in effusion and serum/plasma of cats to diagnose feline infectious peritonitis.一种用于检测猫的积液和血清/血浆中猫冠状病毒突变以诊断猫传染性腹膜炎的实时逆转录聚合酶链反应的敏感性和特异性。
BMC Vet Res. 2017 Aug 2;13(1):228. doi: 10.1186/s12917-017-1147-8.
8
ERDRP-0519 inhibits feline coronavirus in vitro.ERDRP-0519 在体外抑制猫冠状病毒。
BMC Vet Res. 2022 Jan 25;18(1):55. doi: 10.1186/s12917-022-03153-3.
9
Replication of feline coronaviruses in peripheral blood monocytes.猫冠状病毒在外周血单核细胞中的复制
Arch Virol. 2005 Dec;150(12):2483-500. doi: 10.1007/s00705-005-0598-6. Epub 2005 Aug 1.
10
Combination siRNA therapy against feline coronavirus can delay the emergence of antiviral resistance in vitro.针对猫冠状病毒的联合小干扰RNA疗法可在体外延缓抗病毒耐药性的出现。
Vet Microbiol. 2015 Mar 23;176(1-2):10-8. doi: 10.1016/j.vetmic.2014.12.009. Epub 2014 Dec 19.

引用本文的文献

1
Feline Infectious Peritonitis: European Advisory Board on Cat Diseases Guidelines.猫传染性腹膜炎：欧洲猫病咨询委员会指南。
Viruses. 2023 Aug 31;15(9):1847. doi: 10.3390/v15091847.
2
Feline Coronavirus Antivirals: A Review.猫冠状病毒抗病毒药物：综述
Pathogens. 2021 Sep 7;10(9):1150. doi: 10.3390/pathogens10091150.
3
Current status on treatment options for feline infectious peritonitis and SARS-CoV-2 positive cats.猫传染性腹膜炎和 SARS-CoV-2 阳性猫的治疗选择现状。

本文引用的文献

1
A biodegradable polymersome containing Bcl-xL siRNA and doxorubicin as a dual delivery vehicle for a synergistic anticancer effect.一种载有 Bcl-xL siRNA 和阿霉素的可生物降解聚合物囊泡作为协同抗癌作用的双重递药载体。
Macromol Biosci. 2013 Jun;13(6):745-54. doi: 10.1002/mabi.201200448. Epub 2013 Apr 23.
2
Intrahost diversity of feline coronavirus: a consensus between the circulating virulent/avirulent strains and the internal mutation hypotheses?猫冠状病毒的宿主体内多样性：流行的强毒株/无毒株与内部突变假说之间的共识？
ScientificWorldJournal. 2013 Mar 27;2013:572325. doi: 10.1155/2013/572325. Print 2013.
3
Attenuation of influenza virus infectivity with herbal-marine compound (HESA-A): an in vitro study in MDCK cells.
Vet Q. 2020 Dec;40(1):322-330. doi: 10.1080/01652176.2020.1845917.
4
Enhancing the Therapeutic Delivery of Oligonucleotides by Chemical Modification and Nanoparticle Encapsulation.通过化学修饰和纳米颗粒包封来增强寡核苷酸的治疗传递。
Molecules. 2017 Oct 13;22(10):1724. doi: 10.3390/molecules22101724.
5
Expression profiles of immune mediators in feline Coronavirus-infected cells and clinical samples of feline Coronavirus-positive cats.猫冠状病毒感染细胞及猫冠状病毒阳性猫临床样本中免疫介质的表达谱
BMC Vet Res. 2017 Apr 7;13(1):92. doi: 10.1186/s12917-017-1019-2.
草药-海洋化合物（HESA-A）对流感病毒感染力的衰减作用：在 MDCK 细胞中的体外研究。
Virol J. 2012 Feb 16;9:44. doi: 10.1186/1743-422X-9-44.
4
Structural diversity repertoire of gene silencing small interfering RNAs.基因沉默小干扰 RNA 的结构多样性库。
Nucleic Acid Ther. 2011 Jun;21(3):125-31. doi: 10.1089/nat.2011.0286.
5
In vitro inhibition of feline coronavirus replication by small interfering RNAs.小干扰 RNA 对猫冠状病毒复制的体外抑制作用。
Vet Microbiol. 2011 Jun 2;150(3-4):220-9. doi: 10.1016/j.vetmic.2011.01.023. Epub 2011 Feb 1.
6
Triplex forming oligonucleotides against type α1(I) collagen attenuates liver fibrosis induced by bile duct ligation.三链体寡核苷酸靶向 α1(I)型胶原可减轻胆管结扎诱导的肝纤维化。
Biochem Pharmacol. 2010 Dec 1;80(11):1718-26. doi: 10.1016/j.bcp.2010.08.020. Epub 2010 Sep 9.
7
Polyacrylamide gel electrophoresis of RNA.RNA的聚丙烯酰胺凝胶电泳
Cold Spring Harb Protoc. 2010 Jun;2010(6):pdb.prot5444. doi: 10.1101/pdb.prot5444.
8
Antibody-dependent enhancement occurs upon re-infection with the identical serotype virus in feline infectious peritonitis virus infection.在猫传染性腹膜炎病毒感染中，当再次感染相同血清型病毒时会发生抗体依赖性增强现象。
J Vet Med Sci. 2008 Dec;70(12):1315-21. doi: 10.1292/jvms.70.1315.
9
Inhibition of human prostate cancer xenograft growth by 125I labeled triple-helix forming oligonucleotide directed against androgen receptor.125I标记的针对雄激素受体的三链形成寡核苷酸对人前列腺癌异种移植瘤生长的抑制作用
Chin Med J (Engl). 2008 Nov 20;121(22):2284-9.
10
Treatment of cats with feline infectious peritonitis.猫传染性腹膜炎的猫治疗方法。
Vet Immunol Immunopathol. 2008 May 15;123(1-2):172-5. doi: 10.1016/j.vetimm.2008.01.026. Epub 2008 Jan 19.