Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Virol J. 2012 Feb 16;9:44. doi: 10.1186/1743-422X-9-44.
The influenza virus is still one of the most important respiratory risks affecting humans which require effective treatments. In this case, traditional medications are of interest. HESA-A is an active natural biological compound from herbal-marine origin. Previous studies have reported that the therapeutic properties of HESA-A are able to treat psoriasis vulgaris and cancers. However, no antiviral properties have been reported.
This study was designed to investigate the potential antiviral properties of HESA-A and its effects in modulating TNF-α and IL-6 cytokine levels. HESA-A was prepared in normal saline as a stock solution (0.8 mg/ml, pH = 7.4). Percentages of cell survival when exposed to different concentrations of HESA-A at different time intervals was determined by MTT assay. To study the potential antiviral activity of HESA-A, Madin-Darby Canine Kidney (MDCK) cells were treated with the effective concentration (EC50) of HESA-A (0.025 mg/ml) and 100 TCID50/0.1 ml of virus sample under different types of exposure.
Based on the MTT method and hemagglutination assay (HA), HESA-A is capable of improving cell viability to 31% and decreasing HA titre to almost 99% in co-penetration exposures. In addition, based on quantitative real-time PCR (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA), it was found that HESA-A causes decrements in TNF-α and IL-6 cytokine expressions, which was significant for TNF-α (p ≤ 0.05) but not for IL-6.
In conclusion, HESA-A was effective against influenza infection through suppressing cytokine expression.
流感病毒仍然是影响人类的最重要的呼吸道风险之一，需要有效的治疗方法。在这种情况下，传统药物受到关注。HESA-A 是一种源自草药-海洋的活性天然生物化合物。先前的研究报告称，HESA-A 的治疗特性能够治疗寻常型银屑病和癌症。但是，尚未报道其抗病毒特性。
本研究旨在研究 HESA-A 的潜在抗病毒特性及其调节 TNF-α 和 IL-6 细胞因子水平的作用。将 HESA-A 在生理盐水（pH = 7.4）中制备为储备溶液（0.8mg/ml）。通过 MTT 测定法确定在不同浓度的 HESA-A 下暴露于不同时间间隔时细胞的存活率百分比。为了研究 HESA-A 的潜在抗病毒活性，用有效浓度（EC50）的 HESA-A（0.025mg/ml）和 100TCID50/0.1ml 病毒样品处理 Madin-Darby 犬肾（MDCK）细胞在不同类型的暴露下。
根据 MTT 法和血凝（HA）测定法，HESA-A 能够将细胞活力提高到 31％，并将 HA 滴度降低到几乎 99％的共渗透暴露。此外，根据实时定量 PCR（qRT-PCR）和酶联免疫吸附测定（ELISA），发现 HESA-A 导致 TNF-α 和 IL-6 细胞因子表达减少，对 TNF-α 有显著影响（p≤0.05），但对 IL-6 没有影响。
总之，HESA-A 通过抑制细胞因子表达对流感感染有效。
