Jones P B, Vernon M W, Muse K N, Curry T E
Department of Biochemistry, University of Kentucky, Albert B. Chandler Medical Center, Lexington 40536-0084.
J Clin Endocrinol Metab. 1989 Jun;68(6):1039-45. doi: 10.1210/jcem-68-6-1039.
To evaluate the roles of plasminogen activator (PA) and PA inhibitor (PAI) in human ovulation, we obtained follicular fluid and granulosa cells from individual preovulatory follicles of patients undergoing gamete intrafallopian tube transfer. The follicular fluid samples (n = 25) were analyzed for total tissue-type PA antigen, PA enzyme activity by fibrin autography, PAI activity, PAI type 1 (PAI-1) antigen, and PAI-1 mRNA. The follicular fluid of preovulatory follicles contained low levels of total tissue-type PA antigen (less than 1 ng/mL). Fibrin autography experiments indicated little or no detectable PA activity associated with free or unbound PA. The results of the PAI activity assay and PAI-1 antigen determination support the concept of a relative abundance of PAI compared with PA. Hybridization analysis was used to measure the relative amounts of granulosa cell PAI-1 mRNA. The levels of PAI-1 mRNA correlate with follicular fluid PAI concentrations in individual follicles. Taken together, these results support the idea that there is very little free, or active, PA in follicular fluid of human preovulatory follicles, but there is an abundance of PAI. Furthermore, PAI-1 produced by the granulosa cells may represent a major form of PAI in follicular fluid.
为评估纤溶酶原激活物（PA）和PA抑制剂（PAI）在人类排卵中的作用，我们从接受配子输卵管内移植患者的单个排卵前卵泡中获取了卵泡液和颗粒细胞。对25份卵泡液样本进行了总组织型PA抗原、通过纤维蛋白自显影法检测的PA酶活性、PAI活性、1型PAI（PAI - 1）抗原以及PAI - 1 mRNA的分析。排卵前卵泡的卵泡液中总组织型PA抗原水平较低（低于1 ng/mL）。纤维蛋白自显影实验表明，与游离或未结合的PA相关的可检测到的PA活性很少或没有。PAI活性测定和PAI - 1抗原测定的结果支持了与PA相比PAI相对丰富的概念。杂交分析用于测量颗粒细胞PAI - 1 mRNA的相对量。单个卵泡中PAI - 1 mRNA的水平与卵泡液中PAI浓度相关。综上所述，这些结果支持这样一种观点，即人类排卵前卵泡的卵泡液中游离的或有活性的PA极少，但PAI丰富。此外，颗粒细胞产生的PAI - 1可能是卵泡液中PAI的主要形式。
