Jones P B, Muse K N, Wilson E A, Curry T E
Department of Biochemistry, University of Kentucky, Albert B. Chandler Medical Center, Lexington 40536-0084.
J Clin Endocrinol Metab. 1988 Oct;67(4):857-60. doi: 10.1210/jcem-67-4-857.
Studies with rat ovarian cells indicate that proteolytic enzymes, such as plasminogen activator (PA), play a role in the tissue remodeling that occurs before ovulation. In the rat, gonadotropins appear to increase granulosa cell tissue-type plasminogen activator (t-PA) content by increasing the cellular concentration of t-PA mRNA as well as by modulating the activity of a specific PA inhibitor (PAI). We obtained granulosa cells from preovulatory follicles of women undergoing either in vitro fertilization or gamete intra-fallopian tube transfer in order to evaluate the roles of PA and PAI in human ovulation. Samples of granulosa cell total RNA were hybridized with probes for t-PA, urokinase-type PA, PAI type 1 (PAI-1), or inhibin A-chain (as a control). Northern analyses revealed that the RNA of granulosa cells from preovulatory follicles contained little or no detectable PA mRNA. In contrast, two species of PAI mRNA were detected in relative abundance. The signal intensity produced by the PAI-1 probe varied by about 8-fold among patient samples, suggesting that PAI-1 may be useful as a marker of follicular maturation and differentiation. These results demonstrate that human granulosa cells collected immediately before ovulation contain PA inhibitor mRNA, yet have little or no PA mRNA.
对大鼠卵巢细胞的研究表明，蛋白水解酶，如纤溶酶原激活剂（PA），在排卵前发生的组织重塑过程中发挥作用。在大鼠中，促性腺激素似乎通过增加组织型纤溶酶原激活剂（t-PA）mRNA的细胞浓度以及调节特定PA抑制剂（PAI）的活性来增加颗粒细胞中t-PA的含量。我们从接受体外受精或配子输卵管内移植的女性的排卵前卵泡中获取颗粒细胞，以评估PA和PAI在人类排卵中的作用。颗粒细胞总RNA样本与t-PA、尿激酶型PA、1型PAI（PAI-1）或抑制素A链（作为对照）的探针杂交。Northern分析显示，排卵前卵泡颗粒细胞的RNA中几乎检测不到PA mRNA或含量极少。相比之下，检测到两种相对丰富的PAI mRNA。PAI-1探针产生的信号强度在患者样本中变化约8倍，这表明PAI-1可能可用作卵泡成熟和分化的标志物。这些结果表明，排卵前立即采集的人类颗粒细胞含有PA抑制剂mRNA，但PA mRNA极少或没有。
