Eberlin Livia S, Mulcahy John V, Tzabazis Alexander, Zhang Jialing, Liu Huwei, Logan Matthew M, Roberts Heather J, Lee Gordon K, Yeomans David C, Du Bois Justin, Zare Richard N
Department of Chemistry, Stanford University , Stanford, California 94305-5080, United States.
J Am Chem Soc. 2014 Apr 30;136(17):6401-5. doi: 10.1021/ja501635u. Epub 2014 Apr 17.
Determining permeability of a given compound through human skin is a principal challenge owing to the highly complex nature of dermal tissue. We describe the application of an ambient mass spectrometry imaging method for visualizing skin penetration of sodium channel modulators, including novel synthetic analogs of natural neurotoxic alkaloids, topically applied ex vivo to human skin. Our simple and label-free approach enables successful mapping of the transverse and lateral diffusion of small molecules having different physicochemical properties without the need for extensive sample preparation.
由于皮肤组织高度复杂的性质，确定给定化合物透过人体皮肤的渗透性是一项主要挑战。我们描述了一种常压质谱成像方法的应用，该方法用于可视化钠通道调节剂的皮肤渗透情况，这些调节剂包括天然神经毒性生物碱的新型合成类似物，它们以离体方式局部应用于人体皮肤。我们这种简单且无需标记的方法能够成功绘制具有不同物理化学性质的小分子的横向和纵向扩散图谱，而无需进行大量的样品制备。
