Cheung R C, Robinson W S, Marion P L, Greenberg H B
Division of Gastroenterology, Stanford University School of Medicine, California 94305-5701.
J Virol. 1989 Jun;63(6):2445-51. doi: 10.1128/JVI.63.6.2445-2451.1989.
In this article we report the first topological mapping of neutralizing epitopes of a hepadnavirus. Duck hepatitis B virus is the only hepadnavirus that can replicate and spread from cell to cell in tissue culture. As a result, it is possible to study hepadnaviral neutralization in vitro with this system. To accomplish this goal, we produced a library of monoclonal antibodies against duck hepatitis B virus and identified 12 neutralizing monoclonal antibodies by using an in vitro neutralization assay. The characteristics of six of the neutralizing monoclonal antibodies were further studied by epitope mapping. From the results of competitive binding studies, three distinct neutralizing epitopes were identified on the pre-S polypeptides and one was identified on the S polypeptide. Our findings suggest that antibodies to both the pre-S and S gene products of duck hepatitis B virus can neutralize viral infection in vitro. The pre-S gene product is at least as important as the S gene product in eliciting neutralizing antibodies.
在本文中，我们报告了嗜肝DNA病毒中和表位的首次拓扑图谱。鸭乙型肝炎病毒是唯一能在组织培养中进行细胞间复制和传播的嗜肝DNA病毒。因此，利用该系统在体外研究嗜肝DNA病毒中和作用成为可能。为实现这一目标，我们制备了针对鸭乙型肝炎病毒的单克隆抗体文库，并通过体外中和试验鉴定出12种中和单克隆抗体。通过表位作图进一步研究了其中6种中和单克隆抗体的特性。从竞争性结合研究结果来看，在pre-S多肽上鉴定出3个不同的中和表位，在S多肽上鉴定出1个。我们的研究结果表明，针对鸭乙型肝炎病毒pre-S和S基因产物的抗体均可在体外中和病毒感染。在引发中和抗体方面，pre-S基因产物至少与S基因产物同样重要。
