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盐胁迫诱导脂质合成,以在混合营养微藻的双重培养模式下利用生物柴油。

Salinity stress induced lipid synthesis to harness biodiesel during dual mode cultivation of mixotrophic microalgae.

机构信息

Bioengineering and Environmental Centre (BEEC), CSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500 007, India.

Bioengineering and Environmental Centre (BEEC), CSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500 007, India.

出版信息

Bioresour Technol. 2014 Aug;165:288-94. doi: 10.1016/j.biortech.2014.02.103. Epub 2014 Mar 5.

DOI:10.1016/j.biortech.2014.02.103
PMID:24709529
Abstract

Influence of salinity as a stress factor to harness biodiesel was assessed during dual mode cultivation of microalgae by integrating biomass growth phase (BGP) and salinity induced lipid induction phase (LIP). BGP was evaluated in mixotrophic mode employing nutrients (NPK) and carbon (glucose) source while LIP was operated under stress environment with varying salt concentrations (0, 0.5, 1 and 2gNaCl/l). Salinity stress triggered both biomass growth and lipid synthesis in microalgae significantly. BGP showed higher increments in biomass growth (2.55g/l) while LIP showed higher lipid productivity (1gNaCl/l; total/neutral lipid, 23.4/9.2%) than BGP (total/neutral lipid, 15.2/6%). Lower concentrations of salinity showed positive influence on the process while higher concentrations showed marked inhibition. Salinity stress also facilitated in maintaining saturated fatty acid methyl esters in higher amounts which associates with the improved fuel properties. Efficient wastewater treatment was observed during BGP operation indicating the assimilation of carbon/nutrients by microalgae.

摘要

评估了盐度作为胁迫因子对微藻双模式培养生物柴油的影响,该双模式培养将生物量生长阶段(BGP)和盐度诱导的脂类诱导阶段(LIP)相结合。在混合营养模式下利用营养物质(NPK)和碳(葡萄糖)源评估 BGP,而在具有不同盐浓度(0、0.5、1 和 2gNaCl/l)的胁迫环境下进行 LIP 操作。盐度胁迫显著触发微藻的生物量生长和脂类合成。BGP 显示出更高的生物量生长增量(2.55g/l),而 LIP 显示出更高的脂类生产力(1gNaCl/l;总/中性脂,23.4/9.2%)比 BGP(总/中性脂,15.2/6%)。较低浓度的盐度对该过程有积极影响,而较高浓度则表现出明显的抑制作用。盐度胁迫还有助于保持更多的饱和脂肪酸甲酯,这与改善的燃料性能有关。在 BGP 运行期间观察到高效的废水处理,表明微藻对碳/养分的同化。

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