Bioengineering and Environmental Centre (BEEC), CSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500 607, India.
Bioresour Technol. 2013 Aug;142:278-86. doi: 10.1016/j.biortech.2013.05.001. Epub 2013 May 10.
Effect of nutritional mode viz., photoautotrophic, photoheterotrophic and photomixotrophic on the biomass growth and lipid productivity of microalgae was studied. Experiments were designed and operated in biphasic mode i.e., growth phase (GP) followed by stress induced starvation phase (SP). Nutritional mode documented marked influence on biomass growth and subsequent lipid productivity. Mixotrophic mode of operation showed higher biomass growth (4.45 mg/ml) during growth phase while higher lipid productivity was observed with nitrogen deprived autotrophic mode (28.2%) followed by heterotrophic (26.1%) and mixotrophic (19.6%) operations. Relative increments in lipid productivities were noticed in SP operation from GP in mixotrophic operation (2.45) followed by autotrophic (2.2) and heterotrophic (2.14) mode of operations. Higher concentrations of chlorophyll b and presence of lipid accumulating species supported the lipid biosynthesis. Algal fatty acid composition varied with function of nutritional modes and depicted eighteen types of saturated (SFA) and unsaturated fatty acids (USFA) with wide fuel and food characteristics.
研究了营养方式（自养、异养和混合营养）对微藻生物量生长和油脂生产力的影响。实验采用两相模式设计和运行，即生长阶段（GP）后接胁迫诱导饥饿阶段（SP）。营养方式对生物量生长和随后的油脂生产力有显著影响。混合营养操作模式在生长阶段表现出更高的生物量生长（4.45mg/ml），而氮饥饿自养模式（28.2%）表现出更高的油脂生产力，其次是异养（26.1%）和混合营养（19.6%）操作。在 SP 操作中，从 GP 观察到混合营养操作中的油脂生产力相对增加（2.45），其次是自养（2.2）和异养（2.14）操作模式。较高浓度的叶绿素 b 和存在的脂质积累物种支持脂质生物合成。藻类脂肪酸组成随营养方式的不同而变化，显示出十八种饱和脂肪酸（SFA）和不饱和脂肪酸（USFA），具有广泛的燃料和食品特性。
