Slavinskiĭ A A
Lab Delo. 1989(4):32-4.
A method is suggested for scintillating activated neutrophils and monocytes by the intensity of their own fluorescence after incubation with nitroblue tetrazolium (NBT). Only NBT reducing, i.e. activated, phagocytes of the blood and purulent wounds fluorescence. Two types of fluorescence have been observed. Blue fluorescence, induced by UV irradiation, with a maximum within the range of 450-470 nm, is due to reduced pyridine nucleotides and is characteristic of dormant cells. Yellow-green fluorescence evoked by visible light, its maximum being observed at 535 nm, is induced by oxidized flavoproteins and is typical of the cells developing active metabolism. The ratio between cytofluorometrically measured fluorescence intensities of oxidized flavoproteins and reduced pyridine nucleotides is the energy indicator of phagocytosis activation.
提出了一种通过与硝基蓝四氮唑（NBT）孵育后中性粒细胞和单核细胞自身荧光强度来闪烁激活它们的方法。只有血液和脓性伤口中能够还原NBT的吞噬细胞，即被激活的吞噬细胞才会发出荧光。观察到两种类型的荧光。紫外线照射诱导的蓝色荧光，其最大值在450 - 470nm范围内，是由于吡啶核苷酸还原所致，是休眠细胞的特征。可见光激发的黄绿色荧光，其最大值在535nm处，是由氧化型黄素蛋白诱导的，是具有活跃代谢的细胞所特有的。氧化型黄素蛋白和还原型吡啶核苷酸的细胞荧光强度测量比值是吞噬作用激活的能量指标。
