Shono N I, Baskaeva E M
Lab Delo. 1989(4):4-7.
This method is based on Coomassie Brilliant Blue G-250 binding with proteins. The mechanism of protein molecule binding with the stain is described. The authors emphasize high sensitivity of this simple and rapid method and discuss its advantages and shortcomings vs. Lowry's method. The range of laboratory application of the method is considered.
该方法基于考马斯亮蓝G - 250与蛋白质的结合。文中描述了蛋白质分子与染色剂结合的机制。作者强调了这种简单快速方法的高灵敏度,并讨论了其与洛瑞方法相比的优缺点。还考虑了该方法的实验室应用范围。