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三种蛋白质测量方法的比较:应用于支气管肺泡灌洗液

Comparison of three methods for protein measurement: application to bronchoalveolar lavage fluids.

作者信息

Scharfman A, Hayem A, Lebas J, Laine A, Sablonnière B

出版信息

Bull Eur Physiopathol Respir. 1980 Nov-Dec;16(6):785-9.

PMID:7448468
Abstract

The protein contents of bronchoalveolar lavage fluids from normal subjects and patients were estimated by three methods: LOWRY's assay, Coomassie blue method and the automatic laser nephelometric assay. The assays were carried out with native fluids and human serumalbumin as the standard. The lowest values were usually obtained with the dye-binding method. The best correlation coefficients between the assays were obtained from the pulmonary lavage of patients.

摘要

采用三种方法对正常受试者和患者支气管肺泡灌洗液中的蛋白质含量进行了测定

洛瑞法、考马斯亮蓝法和自动激光散射比浊法。以天然液体和人血清白蛋白作为标准品进行测定。通常染料结合法得到的数值最低。各测定方法之间最佳的相关系数来自患者的肺灌洗样本。

相似文献

1
Comparison of three methods for protein measurement: application to bronchoalveolar lavage fluids.三种蛋白质测量方法的比较:应用于支气管肺泡灌洗液
Bull Eur Physiopathol Respir. 1980 Nov-Dec;16(6):785-9.
2
[Indexing protein determinations in bronchoalveolar lavage fluids to a reference substance. Value of alveolar potassium].[支气管肺泡灌洗流体中蛋白质测定对标参考物质。肺泡钾的价值]
Pathol Biol (Paris). 1982 Oct;30(8):711-3.
3
Quantification of proteins in tracheobronchoalveolar lavage fluid by isoelectric focusing and scanning densitometry. A methodological study on rats.通过等电聚焦和扫描密度测定法对气管支气管肺泡灌洗液中的蛋白质进行定量分析。一项关于大鼠的方法学研究。
Eur J Respir Dis Suppl. 1984;138:1-134.
4
Investigating paediatric airways by non-bronchoscopic lavage: normal cellular data.通过非支气管镜灌洗研究小儿气道:正常细胞数据
Clin Exp Allergy. 1996 Jul;26(7):799-806.
5
[Proteins in bronchoalveolar lavage fluid].
Ann Biol Clin (Paris). 1982;40(3):231-3.
6
[Proteins and enzymes in normal human bronchial washing fluids (author's transl)].正常人支气管冲洗液中的蛋白质和酶(作者译)
Bull Eur Physiopathol Respir. 1979 Jul-Aug;15(4):611-24.
7
[Determination of proteins with the Coomassie brilliant blue G 250 method. IV. Use with cerebrospinal fluid proteins and comparative analysis with the biuret and Lowry methods].[考马斯亮蓝G 250法测定蛋白质。IV. 用于脑脊液蛋白质及与双缩脲法和洛瑞法的比较分析]
Boll Soc Ital Biol Sper. 1980 Mar 15;56(5):463-7.
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[Bradford's method of determining protein: application, advantages and disadvantages].[布拉德福德蛋白质测定法:应用、优点与缺点]
Lab Delo. 1989(4):4-7.
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[Bronchoalveolar lavage: a new method in pulmonary diseases].
Rev Paul Med. 1983 May-Jun;101(3):107-8.
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[Nephelometric immunoassay of albumin and IgG in bronchoalveolar lavage fluid (author's transl)].
Nihon Kyobu Shikkan Gakkai Zasshi. 1981 Apr;19(4):230-8.

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