Buttachon Suradet, Arikit Siwaret, Nuchchanart Wirawan, Puangmalee Thanapol, Duanchay Tidapa, Jampameung Nattaya, Sanguansub Sunisa
Department of Entomology, Faculty of Agriculture at Kamphaeng Saen, Kamphaeng Saen Campus, Kasetsart University, Nakhon Pathom 73140, Thailand.
Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kamphaeng Saen Campus, Kasetsart University, Nakhon Pathom 73140, Thailand.
Insects. 2022 Nov 5;13(11):1022. doi: 10.3390/insects13111022.
One of the most impactful pests in several coconut production regions across the world is the coconut mite, Keifer. Scholars can obtain some necessary biogeographic information about coconut mites from studies that explore the geographic patterns of morphological variations and molecular properties among coconut mite populations from various locales. To investigate the geographical origin, ancestral host associations, and colonization history of the mite in Thailand, we obtained DNA sequence data from two mitochondrial (16s and COI) and one nuclear region (ITS) from coconut mite samples originating from 25 populations; additionally, we analyzed the morphological variations in the prodorsal shield and the coxigenital and ventral regions of the mite idiosoma. From the results of experiments using both identification methods, we identified the mite as the coconut mite, (Acari: Eriophyidae). According to the phylogenetic analysis results of the 25 mite samples, we classified the mites as being closely related to mites found by the authors of a previous report in India. We are the first to report the results of a geometric morphometric analysis and molecular identification of in Thailand, and our findings support the idea that the mites' origin and invasion history are not well documented, which makes it difficult to apply quarantine procedures and search for biological pest control agents.
全球多个椰子产区最具影响力的害虫之一是椰子瘿螨，即凯氏瘿螨。学者们可以从一些研究中获取有关椰子瘿螨的必要生物地理信息，这些研究探索了来自不同地区的椰子瘿螨种群的形态变异和分子特性的地理模式。为了调查泰国这种瘿螨的地理起源、原始宿主关联和定殖历史，我们从来自25个种群的椰子瘿螨样本中获取了两个线粒体区域（16s和COI）和一个核区域（ITS）的DNA序列数据；此外，我们分析了瘿螨前盾板以及躯体的生殖腹板和腹面区域的形态变异。根据使用这两种鉴定方法的实验结果，我们将这种瘿螨鉴定为椰子瘿螨（蜱螨亚纲：瘿螨科）。根据对25个瘿螨样本的系统发育分析结果，我们将这些瘿螨归类为与之前印度一份报告的作者所发现的瘿螨密切相关。我们首次报告了泰国椰子瘿螨的几何形态测量分析和分子鉴定结果，我们的研究结果支持这样一种观点，即瘿螨的起源和入侵历史没有得到充分记录，这使得难以应用检疫程序和寻找生物害虫控制剂。
