Lew A M, Langford C J, Anders R F, Kemp D J, Saul A, Fardoulys C, Geysen M, Sheppard M
Walter and Eliza Hall Institute of Medical Research, Victoria, Australia.
Proc Natl Acad Sci U S A. 1989 May;86(10):3768-72. doi: 10.1073/pnas.86.10.3768.
The monoclonal antibody 5C10/66 was shown to afford strong protection in mice against fulminating Plasmodium chabaudi adami infection. This was remarkable, as immunity to this organism is regarded to be mainly T-cell mediated. This antibody identified a 250-kDa molecule in schizonts and an 83-kDa fragment in merozoites. A cDNA clone selected by 5C10/66 was the homologue of the Plasmodium falciparum precursor to the major merozoite surface antigen (PMMSA). Comparison with the P. falciparum sequence showed that the P. chabaudi adami clone encoded the middle portion of the gene and that it can also be divided into variable and conserved blocks. Screening of a set of all possible octamer peptides predicted by the cDNA clone revealed that the core epitope of 5C10/66 was Glu-Thr-Thr-Glu-Thr. This region resides in a variable block of PMMSA.
单克隆抗体5C10/66在小鼠实验中显示出对暴发性查巴迪疟原虫感染具有强大的保护作用。这一点很显著，因为针对这种生物体的免疫被认为主要是由T细胞介导的。该抗体在裂殖体中识别出一个250 kDa的分子，在裂殖子中识别出一个83 kDa的片段。通过5C10/66筛选出的一个cDNA克隆是恶性疟原虫主要裂殖子表面抗原（PMMSA）前体的同源物。与恶性疟原虫序列比较表明，查巴迪疟原虫克隆编码该基因的中间部分，并且它也可分为可变区和保守区。对由该cDNA克隆预测的所有可能的八聚体肽进行筛选，结果显示5C10/66的核心表位为Glu-Thr-Thr-Glu-Thr。该区域位于PMMSA的可变区内。
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