Rajasekharan Satish Kumar, Ramesh Samiraj, Bakkiyaraj Dhamodharan
J Chemother. 2015 Aug;27(4):246-9. doi: 10.1179/1973947814Y.0000000186. Epub 2014 Apr 8.
Candida biofilms on various implanted medical devices and living tissues are serious concern in several hospital-acquired infections. The study was conducted to employ a new therapeutic strategy to inhibit the formation of Candida tropicalis biofilms. No significant antifungal or antibiofilm activity was observed when the sodium butyrate (SB), quercetin, and kaempferol were used as a lone drug. Significant decline (P<0·05) in biofilm formation was noted when sub-lethal concentration of SB (30 mM) was used in combination with the flavonoids (450 μg/ml). Z-stack analysis using 3D-confocal laser scanning microscopy (CLSM) also showed substantial reduction in the biofilm thickness in treated glass slides. In conclusion, this is the first report to our knowledge on implementing a combination therapy using flavonoids and histone deacetylase (HDAC) inhibitor.
各种植入式医疗设备和活体组织上的念珠菌生物膜是几种医院获得性感染中的严重问题。本研究旨在采用一种新的治疗策略来抑制热带念珠菌生物膜的形成。当单独使用丁酸钠（SB）、槲皮素和山奈酚时，未观察到显著的抗真菌或抗生物膜活性。当使用亚致死浓度的SB（30 mM）与类黄酮（450 μg/ml）联合使用时，生物膜形成显著下降（P<0·05）。使用三维共聚焦激光扫描显微镜（CLSM）进行的Z轴堆叠分析也显示，处理过的载玻片上生物膜厚度大幅降低。总之，据我们所知，这是关于使用类黄酮和组蛋白脱乙酰酶（HDAC）抑制剂进行联合治疗的首份报告。
