Kolouchová Irena, Maťátková Olga, Paldrychová Martina, Kodeš Zdeněk, Kvasničková Eva, Sigler Karel, Čejková Alena, Šmidrkal Jan, Demnerová Kateřina, Masák Jan
Department of Biotechnology, University of Chemistry and Technology, Technická 5, 166 28, Prague, Czech Republic.
Institute of Microbiology, CAS, Vídeňská 1083, 142 20, Prague, Czech Republic.
Folia Microbiol (Praha). 2018 May;63(3):261-272. doi: 10.1007/s12223-017-0549-0. Epub 2017 Oct 2.
Microbial adhesion to surfaces and the subsequent biofilm formation may result in contamination in food industry and in healthcare-associated infections and may significantly affect postoperative care. Some plants produce substances with antioxidant and antimicrobial properties that are able to inhibit the growth of food-borne pathogens. The aim of our study was to evaluate antimicrobial and anti-biofilm effect of baicalein, resveratrol, and pterostilbene on Candida albicans, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Escherichia coli. We determined the minimum inhibitory concentrations (MIC), the minimum adhesion inhibitory concentration (MAIC), and the minimum biofilm eradication concentration (MBEC) by crystal violet and XTT determination. Resveratrol and pterostilbene have been shown to inhibit the formation of biofilms as well as to disrupt preformed biofilms. Our results suggest that resveratrol and pterostilbene appear potentially very useful to control and inhibit biofilm contaminations by Candida albicans, Staphylococcus epidermidis, and Escherichia coli in the food industry.
微生物对表面的粘附以及随后的生物膜形成可能导致食品工业中的污染和医疗相关感染，并可能显著影响术后护理。一些植物产生具有抗氧化和抗菌特性的物质，能够抑制食源性病原体的生长。我们研究的目的是评估黄芩素、白藜芦醇和紫檀芪对白色念珠菌、表皮葡萄球菌、铜绿假单胞菌和大肠杆菌的抗菌和抗生物膜作用。我们通过结晶紫和XTT测定法确定了最低抑菌浓度（MIC）、最低粘附抑制浓度（MAIC）和最低生物膜根除浓度（MBEC）。白藜芦醇和紫檀芪已被证明能够抑制生物膜的形成，并破坏预先形成的生物膜。我们的结果表明，白藜芦醇和紫檀芪在控制和抑制食品工业中白色念珠菌、表皮葡萄球菌和大肠杆菌的生物膜污染方面可能非常有用。
