Liman Thomas G, Bachelier-Walenta Katrin, Neeb Lars, Rosinski Jana, Reuter Uwe, Böhm Michael, Endres Matthias
Center for Stroke Research Berlin, Charité - Universitätsmedizin Berlin, Germany Klinik und Poliklinik für Neurologie, Charité - Universitätsmedizin Berlin, Germany
Klinik für Innere Medizin III, Kardiologie, Angiologie und Internistische Intensivmedizin, Universitätsklinikum des Saarlandes, Germany.
Cephalalgia. 2015 Feb;35(2):88-94. doi: 10.1177/0333102414529671. Epub 2014 Apr 8.
Endothelial microparticles (EMPs) are vesicles that are released from activated endothelial cells and serve as a surrogate for endothelial dysfunction (ED). ED may be involved in migraine pathophysiology and contribute to the increased risk of ischemic stroke, particularly in female migraineurs with aura (MA). We sought to determine whether EMPs are elevated in women with MA.
In this case-control study, EMPs were detected by analysing surface markers using fluorescence-activated cell sorting (FACS). Surface markers were measured covering the main cell lines relevant in cardiovascular disease like endothelial cells, platelets, monocytes and leucocytes. Microparticles (MPs) were identified in correlation to calibration by 1 -µm calibrator beads (Beckman Coulter). Arterial stiffness was assessed using fingertip tonometry and the heart rate-adjusted augmentation index (AI).
We included 29 patients with MA and 29 matched controls. MA patients had significantly higher EMPs (CD62E(+)AnnexinV(+): 5142/µl vs 1535/µl; p < 0.001; CD144(+)AnnexinV(+): 6683/µl vs 3107/µl; p < 0.001), monocytic (CD14(+)AnnexinV(+) 6378 vs 3161; p < 0.001), and platelet MPs (CD62P(+)CD42b(+)AnnexinV(+) 5450 vs 3204; p < 0.001). Activated EMPs (CD62E(+)AnnexinV(+)) correlated with heart-rate adjusted AI (r = 0.46; p < 001).
EMP levels are significantly elevated in women with MA and correlated with increased AI. Our findings suggest that endothelial activation is present in women with MA. This might contribute to higher stroke risk in MA.
内皮微粒（EMPs）是从活化内皮细胞释放的囊泡，可作为内皮功能障碍（ED）的替代指标。ED可能参与偏头痛的病理生理过程，并导致缺血性中风风险增加，尤其是有先兆的女性偏头痛患者（MA）。我们旨在确定MA女性患者的EMPs水平是否升高。
在这项病例对照研究中，通过使用荧光激活细胞分选（FACS）分析表面标志物来检测EMPs。测量与心血管疾病相关的主要细胞系（如内皮细胞、血小板、单核细胞和白细胞）的表面标志物。通过1-μm校准微球（贝克曼库尔特公司）与校准物相关联来识别微粒（MPs）。使用指尖血压计和心率校正的增强指数（AI）评估动脉僵硬度。
我们纳入了29例MA患者和29例匹配的对照。MA患者的EMPs水平显著更高（CD62E(+)膜联蛋白V(+)：5142/μl对1535/μl；p<0.001；CD144(+)膜联蛋白V(+)：6683/μl对3107/μl；p<0.001），单核细胞微粒（CD14(+)膜联蛋白V(+) 6378对3161；p<0.001）和血小板微粒（CD62P(+)CD42b(+)膜联蛋白V(+) 5450对3204；p<0.001）。活化的EMPs（CD62E(+)膜联蛋白V(+)）与心率校正的AI相关（r = 0.46；p<0.01）。
MA女性患者的EMPs水平显著升高，并与AI增加相关。我们的研究结果表明MA女性患者存在内皮激活。这可能导致MA患者中风风险更高。
