Department of Nutrition and Health Sciences, Chang Jung Christian University, Tainan 711, Taiwan.
Department of Nutrition, China Medical University, Taichung 404, Taiwan.
J Tradit Complement Med. 2013 Jan;3(1):48-52. doi: 10.4103/2225-4110.106547.
This study investigated effects of the Gynura bicolor (Roxb. and Willd.) DC. ether extract (GBEE) on nitric oxide (NO) and prostaglandin (PG)E2 production on the lipopolysaccharide (LPS)-induced inflammatory response in RAW 264.7 cells. A composition analysis of GBEE showed that the major compounds were b-carotene, chlorophyll, and quercetin, respectively. Furthermore, NO and PGE2 levels of 120 μg/ml GBEE-treated cells were 70% and 9.8%, respectively, than those of cells treated with LPS alone. Immunoblots assays showed that the GBEE dose-dependently suppressed LPS-induced inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 protein levels. The GBEE significantly decreased cytosolic phosphorylated (p)-IκBa and nuclear p65 protein expressions. Electrophoresis mobility shift assays indicated that the GBEE effectively inhibited nuclear factor kappa B (NF-κB) activation induced by LPS. These results support a role of the GBEE in suppressing activation of NF-κB to inhibit NO and PGE2 production in the LPS-induced inflammatory response by RAW 264.7 cells.
本研究探讨了菊三七（Roxb. 和 Willd.）乙醚提取物（GBEE）对脂多糖（LPS）诱导的 RAW 264.7 细胞炎症反应中一氧化氮（NO）和前列腺素（PG）E2 产生的影响。GBEE 的成分分析表明，主要化合物分别为β-胡萝卜素、叶绿素和槲皮素。此外，120μg/ml GBEE 处理的细胞中的 NO 和 PGE2 水平分别比单独用 LPS 处理的细胞高 70%和 9.8%。免疫印迹分析表明，GBEE 呈剂量依赖性地抑制 LPS 诱导的诱导型一氧化氮合酶（iNOS）和环氧化酶（COX）-2 蛋白水平。GBEE 还显著降低细胞质磷酸化（p）-IκBa 和核 p65 蛋白表达。电泳迁移率变动分析表明，GBEE 可有效抑制 LPS 诱导的核因子 kappa B（NF-κB）激活。这些结果支持 GBEE 在抑制 LPS 诱导的 RAW 264.7 细胞炎症反应中 NF-κB 激活以抑制 NO 和 PGE2 产生中的作用。
