Bale Nicole J, Villanueva Laura, Fan Haoxin, Stal Lucas J, Hopmans Ellen C, Schouten Stefan, Sinninghe Damsté Jaap S
Department of Marine Organic Biogeochemistry, NIOZ Royal Netherlands Institute for Sea Research, Den Burg, the Netherlands.
FEMS Microbiol Ecol. 2014 Jul;89(1):99-110. doi: 10.1111/1574-6941.12338. Epub 2014 May 8.
We investigated the occurrence and activity of anaerobic ammonia oxidation (anammox) bacteria in sandy and muddy sand sediments of the southern North Sea. The presence of anammox bacteria was established through the detection of specific phosphocholine-monoether ladderane lipids, 16S rRNA gene, and hydrazine synthase (hzsA) genes. Anammox activity was measured in intact sediment cores (in situ rate) and in sediment slurries (potential rate) as the rate of N2 evolution from (15) N-labeled substrates and compared to the transcriptional activity of genes of anammox bacteria. The contribution of anammox to N2 production ranged between 0% and 29%. The potential rate of anammox agreed well with the abundance of anammox bacteria 16S rRNA and hzsA gene copies and the transcriptional activity of the anammox bacteria 16S rRNA gene. We found a higher abundance and activity of anammox bacteria in sediments with higher organic carbon content and also higher activity in summer than in winter. The abundance of anammox bacteria and their potential anammox rates were similar to those reported for other marine coastal sediments, suggesting that potentially they are important contributors to the nitrogen cycle in sandy sediments of shallow continental shelf areas.
我们研究了北海南部沙质和泥质沙沉积物中厌氧氨氧化（anammox）细菌的存在情况及活性。通过检测特定的磷酸胆碱 - 单醚梯形烷脂质、16S rRNA基因和肼合酶（hzsA）基因来确定厌氧氨氧化细菌的存在。厌氧氨氧化活性在完整沉积物岩芯（原位速率）和沉积物浆液（潜在速率）中进行测量，以（15）N标记底物产生N2的速率为准，并与厌氧氨氧化细菌基因的转录活性进行比较。厌氧氨氧化对N2产生的贡献在0%至29%之间变化。厌氧氨氧化的潜在速率与厌氧氨氧化细菌16S rRNA和hzsA基因拷贝数以及厌氧氨氧化细菌16S rRNA基因的转录活性高度吻合。我们发现，在有机碳含量较高的沉积物中，厌氧氨氧化细菌的丰度和活性更高；而且夏季的活性高于冬季。厌氧氨氧化细菌的丰度及其潜在厌氧氨氧化速率与其他海洋海岸沉积物报告的情况相似,这表明它们可能是浅海陆架区域沙质沉积物中氮循环的重要贡献者。
