McCusker M T, Chung K F, Roberts N M, Barnes P J
Department of Thoracic Medicine, National Heart and Lung Institute, Brompton Hospital, London, England.
J Allergy Clin Immunol. 1989 Jun;83(6):1118-24. doi: 10.1016/0091-6749(89)90455-7.
Topical capsaicin pretreatment is known to deplete cutaneous sensory nerves of neuropeptides. We have assessed the effect of topical capsaicin pretreatment on the responses to intradermal injections of histamine and platelet-activating factor (PAF) in six normal subjects, and of prostaglandin E2, histamine, and antigen in 10 atopic subjects. Capsaicin pretreatment caused significant inhibition of the immediate flare response to histamine in both normal (19.8 +/- 2.6 to 7.3 +/- 2.9 cm2 at 5 minutes; p less than 0.01) and atopic subjects (16.5 +/- 1.4 to 10.3 +/- 1.9 cm2 at 5 minutes; p less than 0.01). The PAF-induced flare was also inhibited from 12.2 +/- 2.9 to 2.7 +/- 1.6 cm2 at 5 minutes after injection (p less than 0.01). In contrast, capsaicin pretreatment did not significantly alter the flare responses to prostaglandin E2 or antigen in atopic subjects. The acute wheal responses to all stimuli were unchanged, as was the late-phase response to antigen. These results support the hypothesis that the cutaneous vasodilator effect of histamine and PAF may be mediated by a local axon reflex involving the release of neuropeptides from sensory nerves. A consistent effect of capsaicin pretreatment on the flare response induced by endogenous mediators released during a cutaneous IgE-mediated response was not observed. Increases in microvascular permeability and the late-phase response to antigen are independent of neuropeptide release from cutaneous nerves.
已知局部应用辣椒素预处理可使皮肤感觉神经中的神经肽耗竭。我们评估了局部应用辣椒素预处理对6名正常受试者皮内注射组胺和血小板活化因子（PAF），以及10名特应性受试者皮内注射前列腺素E2、组胺和抗原后反应的影响。辣椒素预处理可显著抑制正常受试者（5分钟时从19.8±2.6平方厘米降至7.3±2.9平方厘米；p＜0.01）和特应性受试者（5分钟时从16.5±1.4平方厘米降至10.3±1.9平方厘米；p＜0.01）对组胺的即刻红晕反应。PAF诱导的红晕在注射后5分钟也从12.2±2.9平方厘米抑制至2.7±1.6平方厘米（p＜0.01）。相比之下，辣椒素预处理并未显著改变特应性受试者对前列腺素E2或抗原的红晕反应。对所有刺激的急性风团反应均未改变，对抗原的迟发相反应也是如此。这些结果支持以下假说：组胺和PAF的皮肤血管舒张作用可能由涉及感觉神经释放神经肽的局部轴突反射介导。未观察到辣椒素预处理对皮肤IgE介导反应期间释放的内源性介质诱导的红晕反应有一致影响。微血管通透性增加和对抗原的迟发相反应与皮肤神经释放神经肽无关。
