Paradis K, Sharp H L
Department of Pediatrics, University of Minnesota, Minneapolis 55455.
J Lab Clin Med. 1989 Jun;113(6):689-94.
The epithelium of the biliary tree is involved in the response to numerous liver disease processes including immunologic destruction during liver transplant rejection and liver graft-versus-host disease after bone marrow transplantation. Furthermore, very little is known concerning the function of this previously inaccessible epithelium, because attempts to isolate and culture biliary cells have been unsuccessful until recently. We present a method for the isolation and culture of bile ductular cells from mice with external bile duct obstruction. Over 85% of the isolated cells stain positive for cytokeratin 19, which is characteristic of murine biliary epithelium. Incubation with recombinant murine gamma-interferon resulted in increased class II antigen expression on the isolated cell surface. When these cells were placed, on a basement membrane matrix, they formed duct-like structures composed of cells that had the morphologic characteristics of bile ductular epithelium when examined by transmission electron microscopy. The ability to isolate murine biliary epithelium that forms duct-like structures will be useful for the in vitro study of biliary epithelial characteristics and injury.
胆管树的上皮细胞参与多种肝脏疾病过程的反应，包括肝移植排斥反应期间的免疫破坏以及骨髓移植后的肝移植物抗宿主病。此外，关于这种以前难以获取的上皮细胞的功能，人们了解甚少，因为直到最近，分离和培养胆管细胞的尝试都未成功。我们提出了一种从患有肝外胆管梗阻的小鼠中分离和培养胆小管细胞的方法。超过85%的分离细胞细胞角蛋白19染色呈阳性，这是鼠胆管上皮细胞的特征。用重组鼠γ干扰素孵育导致分离细胞表面的II类抗原表达增加。当将这些细胞置于基底膜基质上时，它们形成了由细胞组成的导管样结构，通过透射电子显微镜检查时，这些细胞具有胆小管上皮细胞的形态学特征。分离出能形成导管样结构的鼠胆管上皮细胞的能力将有助于对胆管上皮细胞特征和损伤进行体外研究。
