Abdelhamid Hani Nasser, Wu Hui-Fen
Department of Chemistry, Assuit University, Assuit, 71515, Egypt.
Department of Chemistry and Center of Nanoscience and Nanotechnology, National Sun Yat-Sen University, Kaohsiung, 80424, ROC Taiwan.
Anal Bioanal Chem. 2016 Jul;408(17):4485-502. doi: 10.1007/s00216-016-9374-6. Epub 2016 Mar 14.
Gold nanoparticles (AuNPs) assisted laser desorption/ionization mass spectrometry (GALDI-MS) provided new horizons and offered many functions for various applications. This review summarized AuNPs applications for analytical, biotechnology and proteomics. AuNPs efficiently absorbed the laser radiation and transferred the energy to the analyte for the desorption/ionization process. The unique features of AuNPs such as large surface area and high absorption coefficient lead not only to high resolution, low interference and low limit of detection, but also offered selective detection for certain species. AuNPs provided an excellent surface for the analysis of several species such as small molecules, biomarkers, proteins and cells (pathogenic bacteria or cancer cells). AuNPs played many roles such as surface for LDI-MS, probe and stationary phase for separation or preconcentration. AuNPs modified various surface chemistry was applied for a wide range of different wavelength. AuNPs severed as a source of Au(+) ions that were suitable for analyte cationisation. Characterization of Au nanoclusters (AuNCs) by mass spectrometry, pros and cons were also highlighted. Graphical Abstract Schematic representation of the analysis by Gold Nanoparticles Assisted Laser Desorption/Ionization Mass Spectrometry (GALDI-MS).
金纳米颗粒（AuNPs）辅助激光解吸/电离质谱（GALDI-MS）为各种应用提供了新视野并具备多种功能。本综述总结了AuNPs在分析、生物技术和蛋白质组学方面的应用。AuNPs能有效吸收激光辐射并将能量传递给分析物以进行解吸/电离过程。AuNPs的独特特性，如大表面积和高吸收系数，不仅导致高分辨率、低干扰和低检测限，还能对某些物种进行选择性检测。AuNPs为多种物种的分析提供了出色的表面，如小分子、生物标志物、蛋白质和细胞（病原菌或癌细胞）。AuNPs发挥了许多作用，如作为激光解吸电离质谱的表面、分离或预浓缩的探针和固定相。修饰了各种表面化学性质的AuNPs被应用于广泛的不同波长。AuNPs可作为适用于分析物阳离子化的Au(+)离子源。还重点介绍了通过质谱对金纳米团簇（AuNCs）的表征及其优缺点。图形摘要 金纳米颗粒辅助激光解吸/电离质谱（GALDI-MS）分析的示意图。
