Holzmann H, Mandl C W, Guirakhoo F, Heinz F X, Kunz C
Institute of Virology, University of Vienna, Austria.
J Gen Virol. 1989 Jan;70 ( Pt 1):219-22. doi: 10.1099/0022-1317-70-1-219.
Antigenic variants of tick-borne encephalitis virus were selected by the use of six neutralizing monoclonal antibodies (MAbs), each defining a different epitope of the envelope glycoprotein E. These variants were characterized with respect to antigenic changes by analysing the binding of each of 18 precisely mapped MAbs in ELISA and haemagglutination inhibition tests. The results yielded information about interrelations between epitopes exceeding that obtained previously from competitive binding studies. In addition, variants were tested for their specific haemagglutination activities, which revealed a significant reduction of this functional activity in one of the variants.
通过使用六种中和单克隆抗体（MAb）选择蜱传脑炎病毒的抗原变异体，每种抗体定义包膜糖蛋白E的不同表位。通过在ELISA和血凝抑制试验中分析18种精确定位的MAb各自的结合情况，对这些变异体的抗原变化进行了表征。结果所产生的关于表位之间相互关系的信息超过了先前从竞争性结合研究中获得的信息。此外，还测试了变异体的特异性血凝活性，结果显示其中一个变异体的这种功能活性显著降低。
