Buckland R, Giraudon P, Wild F
Immunovirologie Moléculaire et Cellulaire UM 380030-CNRS, Faculté Alexis Carrel, Lyon, France.
J Gen Virol. 1989 Feb;70 ( Pt 2):435-41. doi: 10.1099/0022-1317-70-2-435.
Three distinct antigenic determinants on the nucleoprotein (NP) of measles virus were localized. These epitopes were defined by three monoclonal antibodies, one of which recognized all measles virus field strains examined, whereas the other two were variable. A measles virus NP cDNA subclone representing 502 of the 525 amino acids was cloned into a bacterial expression vector plasmid (pRIT) and expressed as a Protein A-NP fusion protein in Escherichia coli. The expressed protein reacted with all three monoclonal antibodies. A series of NP gene deletion was constructed in order to locate the antigenic sites. The antigenic site identified on all measles virus strains studied, which was designated site I, was located between amino acids 122 and 150. The two variable epitopes were located at the C terminus of the protein (site II at 457 to 476; site III at 519 to 525). The structural and biological implications of these observations are discussed.
麻疹病毒核蛋白（NP）上三个不同的抗原决定簇被定位。这些表位由三种单克隆抗体定义，其中一种识别所有检测的麻疹病毒野毒株，而另外两种具有变异性。一个代表525个氨基酸中502个氨基酸的麻疹病毒NP cDNA亚克隆被克隆到细菌表达载体质粒（pRIT）中，并在大肠杆菌中表达为蛋白A-NP融合蛋白。表达的蛋白与所有三种单克隆抗体发生反应。构建了一系列NP基因缺失体以定位抗原位点。在所有研究的麻疹病毒株上鉴定出的抗原位点，被命名为位点I，位于氨基酸122和150之间。两个可变表位位于该蛋白的C末端（位点II在457至476；位点III在519至525）。讨论了这些观察结果的结构和生物学意义。
