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Evaluation of hemagglutinin protein-specific immunoglobulin M for diagnosis of measles by an enzyme-linked immunosorbent assay based on recombinant protein produced in a high-efficiency mammalian expression system.基于高效哺乳动物表达系统生产的重组蛋白，通过酶联免疫吸附测定法评估血凝素蛋白特异性免疫球蛋白M用于麻疹诊断的情况。
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Immunosorbent assay based on recombinant hemagglutinin protein produced in a high-efficiency mammalian expression system for surveillance of measles immunity.基于在高效哺乳动物表达系统中产生的重组血凝素蛋白的免疫吸附测定法用于监测麻疹免疫力。
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本文引用的文献

1
Cloning, expression, and purification of the nucleocapsid protein of SARS coronavirus.严重急性呼吸综合征冠状病毒核衣壳蛋白的克隆、表达及纯化
Dokl Biochem Biophys. 2004 Jul-Aug;397(1):239-41. doi: 10.1023/b:dobi.0000039473.88598.16.
2
Comparison of predicted amino acid sequences of measles virus strains in the Edmonston vaccine lineage.埃德蒙斯顿疫苗株系中麻疹病毒株的预测氨基酸序列比较。
J Virol. 2001 Jan;75(2):910-20. doi: 10.1128/JVI.75.2.910-920.2001.
3
Evaluation of different measles IgG assays based on recombinant proteins using a panel of low-titre sera.使用一组低滴度血清对基于重组蛋白的不同麻疹IgG检测方法进行评估。
J Virol Methods. 2000 Feb;84(2):191-200. doi: 10.1016/s0166-0934(99)00143-3.
4
Evaluation of hemagglutinin protein-specific immunoglobulin M for diagnosis of measles by an enzyme-linked immunosorbent assay based on recombinant protein produced in a high-efficiency mammalian expression system.基于高效哺乳动物表达系统生产的重组蛋白，通过酶联免疫吸附测定法评估血凝素蛋白特异性免疫球蛋白M用于麻疹诊断的情况。
J Clin Microbiol. 1998 Dec;36(12):3509-13. doi: 10.1128/JCM.36.12.3509-3513.1998.
5
A simplified immunoassay based on measles virus recombinant hemagglutinin protein for testing the immune status of vaccinees.一种基于麻疹病毒重组血凝素蛋白的简易免疫测定法，用于检测疫苗接种者的免疫状态。
J Virol Methods. 1998 Sep;74(1):77-87. doi: 10.1016/s0166-0934(98)00073-1.
6
Immunosorbent assay based on recombinant hemagglutinin protein produced in a high-efficiency mammalian expression system for surveillance of measles immunity.基于在高效哺乳动物表达系统中产生的重组血凝素蛋白的免疫吸附测定法用于监测麻疹免疫力。
J Clin Microbiol. 1998 Mar;36(3):721-6. doi: 10.1128/JCM.36.3.721-726.1998.
7
Measles virus nucleocapsid protein expressed in insect cells assembles into nucleocapsid-like structures.在昆虫细胞中表达的麻疹病毒核衣壳蛋白组装成核衣壳样结构。
J Gen Virol. 1993 Jul;74 ( Pt 7):1439-44. doi: 10.1099/0022-1317-74-7-1439.
8
Production of measles nucleoprotein in different expression systems and its use as a diagnostic reagent.麻疹核蛋白在不同表达系统中的生产及其作为诊断试剂的应用。
J Virol Methods. 1994 Oct;49(3):257-68. doi: 10.1016/0166-0934(94)90141-4.
9
Expression of the measles virus nucleoprotein gene in Escherichia coli and assembly of nucleocapsid-like structures.
Gene. 1995 Jul 28;160(2):173-8. doi: 10.1016/0378-1119(95)00227-w.
10
The proteins of morbilliviruses.麻疹病毒的蛋白质
J Gen Virol. 1983 Jun;64 (Pt 6):1205-19. doi: 10.1099/0022-1317-64-6-1205.

麻疹病毒重组蛋白及其免疫生物学特性的研究。

Study of measles virus recombinant proteins and their immunobiological properties.

作者信息

Kulak M V, Netesova N A, Belavin P A, Seregina E V, Ignatyev G M

机构信息

Vector State Research Center of Virology and Biotechnology, Kol'tsovo, Novosibirsk oblast, Russia.

出版信息

Mol Gen Microbiol Virol. 2008;23(1):42. doi: 10.1007/s11965-008-1007-2. Epub 2008 Apr 17.

DOI:10.1007/s11965-008-1007-2
PMID:32214646
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7089051/
Abstract

Recombinant proteins rN (nucleocapsid) and rH/Nh (hemagglutinin) of the measles virus strain NovO/96 of genotype A were obtained. The immunobiological properties of the proteins were studied in the reaction with a panel of positive and negative sera. BALB/c mice were immunized with recombinant proteins and native antigen of the measles virus strain NovO/96 in order to obtain hyperimmune serum and its analysis using ELISA (enzyme-linked immunosorbent assay) and PRN (plaque reduction neutralization). The hyperimmune sera against recombinant proteins and native antigen of the measles virus strain NovO/96 were found to be highly active in ELISA. The antibodies against the proteins rN and rH/Nh were found to be capable of neutralizing the virus in titer 1 : 13.5 and 1 : 22.9, respectively. The neutralization titer of the antibodies generated against native antigen of the measles virus strain NovO/96 was 1 : 25.7.

摘要

获得了A基因型麻疹病毒株NovO/96的重组蛋白rN（核衣壳）和rH/Nh（血凝素）。在与一组阳性和阴性血清的反应中研究了这些蛋白的免疫生物学特性。用重组蛋白和麻疹病毒株NovO/96的天然抗原免疫BALB/c小鼠，以获得超免疫血清，并使用酶联免疫吸附测定（ELISA）和蚀斑减少中和试验（PRN）对其进行分析。发现针对麻疹病毒株NovO/96重组蛋白和天然抗原的超免疫血清在ELISA中具有高活性。发现针对蛋白rN和rH/Nh的抗体能够分别以1:13.5和1:22.9的效价中和病毒。针对麻疹病毒株NovO/96天然抗原产生的抗体的中和效价为1:25.7。