Hachinohe S, Sugawara K, Nishimura H, Kitame F, Nakamura K
Department of Bacteriology, Yamagata University School of Medicine, Japan.
J Gen Virol. 1989 May;70 ( Pt 5):1287-92. doi: 10.1099/0022-1317-70-5-1287.
Five monoclonal antibodies (J14, J9, Q5, K16, S16), directed to three distinct antigenic sites (A-1, A-2, B-1) on the haemagglutinin-esterase glycoprotein of influenza C virus, were analysed for their ability to inhibit the receptor-destroying enzyme (RDE) activity of the virus, utilizing various assay systems. The ability of influenza C virus to destroy the receptors on chicken erythrocytes was inhibited efficiently by the antibodies to site A-1 (J14, J9, Q5) but not by those to site A-2 (K16) and sit B-1 (S16). Of the three antibodies to site A-1, J14 showed the highest inhibitory activity. Antibodies to sites A-1 and A-2 inhibited the ability of RDE to inactivate the haemagglutination inhibition activity of rat serum inhibitors, but the highest activity was observed again with J14. Thus the RDE site of influenza C virus may be located closest to the epitope recognized by J14. The removal of O-acetyl groups from either 9-O-acetyl-N-acetylneuraminic acid or p-nitrophenylacetate, caused by the viral RDE, was not prevented at all by any of the monoclonal antibodies tested. Furthermore, none of several polyclonal antiviral sera prepared in different animal species was able to block the hydrolysis of these small substrates, raising the possibility that the catalytic site of influenza C viral RDE is antigenically silent.
针对丙型流感病毒血凝素酯酶糖蛋白上三个不同抗原位点（A - 1、A - 2、B - 1）的五种单克隆抗体（J14、J9、Q5、K16、S16），利用各种检测系统分析了它们抑制该病毒受体破坏酶（RDE）活性的能力。针对A - 1位点（J14、J9、Q5）的抗体能有效抑制丙型流感病毒破坏鸡红细胞上受体的能力，而针对A - 2位点（K16）和B - 1位点（S16）的抗体则不能。在针对A - 1位点的三种抗体中，J14表现出最高的抑制活性。针对A - 1和A - 2位点的抗体抑制了RDE使大鼠血清抑制剂的血凝抑制活性失活的能力，但J14再次表现出最高活性。因此，丙型流感病毒的RDE位点可能最靠近J14识别的表位。病毒RDE引起的从9 - O - 乙酰 - N - 乙酰神经氨酸或对硝基苯乙酸上去除O - 乙酰基的过程，完全没有被任何一种测试的单克隆抗体所阻止。此外，在不同动物物种中制备的几种多克隆抗病毒血清也都不能阻断这些小分子底物的水解，这增加了丙型流感病毒RDE催化位点在抗原上不活跃的可能性。
