Vlasak R, Krystal M, Nacht M, Palese P
Department of Microbiology, Mount Sinai School of Medicine, New York, New York 10029.
Virology. 1987 Oct;160(2):419-25. doi: 10.1016/0042-6822(87)90013-4.
A cDNA copy of RNA segment 4 of influenza C/Cal/78 virus was cloned into an SV40 vector and expressed in CV-1 cells. The gene product expressed from the SV40 recombinant virus was immunoprecipitated by monoclonal antibodies directed against the influenza C virus glycoprotein. Cells infected with the recombinant virus also exhibited C virus-specific hemagglutinin and O-acetylesterase activity. This suggests that the same C virus protein is associated with receptor-binding as well as receptor-destroying activity. The latter viral activity was measured using as substrates bovine submaxillary mucin or a low molecular weight compound p-nitrophenylacetate. In analogy to the parainfluenza virus HN protein, the influenza C virus glycoprotein was termed HE, because it possesses hemagglutinin and esterase (receptor-destroying) activity.
将C/Cal/78流感病毒RNA片段4的cDNA拷贝克隆到SV40载体中，并在CV-1细胞中表达。用针对C型流感病毒糖蛋白的单克隆抗体对SV40重组病毒表达的基因产物进行免疫沉淀。感染重组病毒的细胞也表现出C病毒特异性血凝素和O-乙酰酯酶活性。这表明相同的C病毒蛋白与受体结合以及受体破坏活性有关。后者的病毒活性是用牛颌下粘蛋白或低分子量化合物对硝基苯乙酸作为底物来测定的。与副流感病毒HN蛋白类似，C型流感病毒糖蛋白被称为HE，因为它具有血凝素和酯酶（受体破坏）活性。
