Tsuchida Y, Kaneko M, Fukui M, Sakaguchi H, Ishiguro T
Department of Surgery, National Children's Hospital, Tokyo, Japan.
J Pediatr Surg. 1989 Apr;24(4):350-5. doi: 10.1016/s0022-3468(89)80267-2.
A sensitive new technique for lectin-affinity immunoelectrophoresis was applied to samples from 28 infants and children in order to distinguish the origin of elevated alpha-fetoprotein (AFP) in sera. This new immunoelectrophoresis was successfully performed within 24 hours in sera with AFP as small as 910 ng/mL. With combined use of concanavalin A (Con A) and lentil agglutinin (LCH) binding tests, AFPs were classified into three subtypes: benign hepatic condition type (six patients), hepatocellular carcinoma type (nine patients) and yolk sac type (12 patients). AFP was of hepatocellular carcinoma type in all seven patients with hepatoblastoma, and of benign hepatic condition type in six of seven patients with elevated AFP due to conditions such as hepatitis, biliary atresia, and normal newborn. The question as to whether AFP produced in "hepatoblastoma" is of benign hepatic condition type or hepatocellular carcinoma type was first answered by the information in this present report. The differentiation between yolk sac and general hepatic AFPs was completed with the Con A binding test.
一种用于凝集素亲和免疫电泳的灵敏新技术被应用于28名婴幼儿和儿童的样本，以区分血清中甲胎蛋白（AFP）升高的来源。这种新的免疫电泳在血清中AFP低至910 ng/mL时，能在24小时内成功完成。通过联合使用刀豆球蛋白A（Con A）和扁豆凝集素（LCH）结合试验，AFP被分为三种亚型：良性肝病型（6例患者）、肝细胞癌型（9例患者）和卵黄囊型（12例患者）。在所有7例肝母细胞瘤患者中，AFP均为肝细胞癌型；在7例因肝炎、胆道闭锁和正常新生儿等情况导致AFP升高的患者中，有6例为良性肝病型。本报告中的信息首次回答了“肝母细胞瘤”中产生的AFP是良性肝病型还是肝细胞癌型这一问题。通过Con A结合试验完成了卵黄囊AFP与一般肝脏AFP的区分。
