Ishiguro T, Sakaguchi H, Fukui M, Sugitachi I
Jpn J Surg. 1986 Jan;16(1):16-21. doi: 10.1007/BF02471064.
Using a modified method of concanavalin A (Con A), lentil lectin (LCH) or phytohemagglutinin-E (PHA-E) affinity crossed-line immunoelectrophoresis (ACIE), we studied alpha-fetoprotein (AFP) subfractions in 69 sera, including 58 from patients with primary liver cancer and 11 from patients with hepatic metastasis of gastric cancer. We found that Con A non-reactive subfraction (type b) or LCH weakly-reactive subfraction (type B) was more frequently detected in metastatic liver cancer, as compared with liver cancer hepatoma. The amount of Con A non-reactive subfraction (type b) or of PHA-E reactive subfraction (type X) was significantly higher in case of metastatic liver cancer than in primary liver cancer. Since different affinities between AFP and lectins are due to the microheterogeneity in AFP sugar chain, our findings suggest that AFP in primary liver cancer and metastatic liver cancer is glycosylated in a different manner. It is also indicated that different patterns of AFP subfractions identified by the combination of Con A, LCH or PHA-E ACIE facilitate a differential diagnosis of these hepatic malignancies.
采用改良的伴刀豆球蛋白A（Con A）、扁豆凝集素（LCH）或植物血凝素-E（PHA-E）亲和交叉免疫电泳（ACIE）方法，我们研究了69份血清中的甲胎蛋白（AFP）亚组分，其中包括58份原发性肝癌患者的血清和11份胃癌肝转移患者的血清。我们发现，与肝癌相比，转移性肝癌中更常检测到Con A无反应性亚组分（b型）或LCH弱反应性亚组分（B型）。转移性肝癌中Con A无反应性亚组分（b型）或PHA-E反应性亚组分（X型）的量明显高于原发性肝癌。由于AFP与凝集素之间的不同亲和力是由于AFP糖链的微观异质性所致，我们的研究结果表明，原发性肝癌和转移性肝癌中的AFP糖基化方式不同。还表明，通过Con A、LCH或PHA-E ACIE联合鉴定的AFP亚组分不同模式有助于这些肝脏恶性肿瘤的鉴别诊断。
