Ishiguro T, Sawada M, Sakaguchi H, Sugitachi I
Am J Reprod Immunol (1980). 1984 Apr-May;5(3):102-5. doi: 10.1111/j.1600-0897.1984.tb00296.x.
Using concanavalin A (Con A) crossed-line affinity immunoelectrophoresis and lentil lectin (LCH) crossed-line affinity immunoelectrophoresis, alpha-fetoprotein (AFP) subfractions were studied in sera including three sera from nude mice heterotransplanted with human yolk sac tumor of the ovary and three sera from patients with yolk sac tumor, mature solid teratoma, or immature solid teratoma of the ovary. In sera of nude mice bearing yolk sac tumor or from a patient with yolk sac tumor, subfractions from yolk sac and those from fetal liver were identified. Since AFP subfractions from yolk sac and fetal liver can be differentiated according to the carbohydrate moieties, our findings indicate that AFP produced by yolk sac tumor and fetal yolk sac are to some extent differently glycosylated. We also found that AFP produced by yolk sac tumor and fetal yolk sac are to some extent differently glycosylated. We also found that AFP in both mature and immature solid teratoma was composed of two subfractions ontogenetically originating from yolk sac or fetal liver. All these findings indicate that more than two different factors are responsible for the AFP synthesis in germ cell tumor of the ovary.
利用伴刀豆球蛋白A（Con A）交叉线亲和免疫电泳和扁豆凝集素（LCH）交叉线亲和免疫电泳，对血清中的甲胎蛋白（AFP）亚组分进行了研究，这些血清包括来自异种移植人卵巢卵黄囊瘤的裸鼠的三份血清以及来自患有卵巢卵黄囊瘤、成熟实性畸胎瘤或未成熟实性畸胎瘤患者的三份血清。在携带卵黄囊瘤的裸鼠血清或来自卵黄囊瘤患者的血清中，鉴定出了来自卵黄囊和胎儿肝脏的亚组分。由于来自卵黄囊和胎儿肝脏的AFP亚组分可根据碳水化合物部分进行区分，我们的研究结果表明，卵黄囊瘤产生的AFP和胎儿卵黄囊产生的AFP在某种程度上糖基化不同。我们还发现，卵黄囊瘤产生的AFP和胎儿卵黄囊产生的AFP在某种程度上糖基化不同。我们还发现，成熟和未成熟实性畸胎瘤中的AFP均由两个在个体发生上起源于卵黄囊或胎儿肝脏的亚组分组成。所有这些发现表明，卵巢生殖细胞肿瘤中AFP的合成由两种以上不同因素负责。
