Möller Anja-Maria, Korytář Tomáš, Köllner Bernd, Schmidt-Posthaus Heike, Segner Helmut
Centre for Fish and Wildlife Health, University of Bern, Länggassstrasse 122, CH-3012 Bern, Switzerland; Institute of Immunology, Federal Research Institute for Animal Health, Friedrich-Loeffler-Institut, Südufer 10, 17493 Greifswald-Insel Riems, Germany.
Institute of Immunology, Federal Research Institute for Animal Health, Friedrich-Loeffler-Institut, Südufer 10, 17493 Greifswald-Insel Riems, Germany.
Dev Comp Immunol. 2014 Oct;46(2):518-29. doi: 10.1016/j.dci.2014.03.020. Epub 2014 Apr 6.
The existence of a resident population of intrahepatic immune cells (IHICs) is well documented for mammalian vertebrates, however, it is uncertain whether IHICs are present in the liver of teleostean fish. In the present study we investigated whether trout liver contains an IHIC population, and if so, what the relative cellular composition of this population is. The results provide clear evidence for the existence of an IHIC population in trout liver, which constitutes 15-29% of the non-hepatocytes in the liver, and with a cellular composition different to that of the blood leukocyte population. We also analyzed the response of IHICs to a non-infectious liver challenge with the hepatotoxic and immunotoxic chemical, benzo[a]pyrene (BaP). Juvenile trout were treated with BaP (25 or 100mg/kgbw) at levels sufficient to induce the molecular pathway of BaP metabolism while not causing pathological and inflammatory liver changes. The IHIC population responded to the BaP treatments in a way that differed from the responses of the leukocyte populations in trout blood and spleen, suggesting that IHICs are an independently regulated immune cell population.
哺乳动物脊椎动物肝内免疫细胞（IHICs）常驻群体的存在已有充分记录，然而，硬骨鱼的肝脏中是否存在IHICs尚不确定。在本研究中，我们调查了虹鳟鱼肝脏中是否含有IHIC群体，如果含有，该群体的相对细胞组成是什么。结果为虹鳟鱼肝脏中存在IHIC群体提供了明确证据，该群体占肝脏中非肝细胞的15%-29%，其细胞组成与血液白细胞群体不同。我们还分析了IHICs对具有肝毒性和免疫毒性的化学物质苯并[a]芘（BaP）所致非感染性肝脏刺激的反应。用足以诱导BaP代谢分子途径但不会引起肝脏病理和炎症变化的剂量（25或100mg/kg体重）对幼龄虹鳟鱼进行BaP处理。IHIC群体对BaP处理的反应方式与虹鳟鱼血液和脾脏中白细胞群体的反应不同，这表明IHICs是一个独立调节的免疫细胞群体。
