Tamariz Jesús H, Lezameta Lizet, Guerra Humberto
Universidad Peruana Cayetano Heredia, Lima, Perú
Rev Peru Med Exp Salud Publica. 2014;31(1):69-77.
To assess the bacteriophage activity in localized and systemic infections caused by Staphylococcus aureus resistant to methicilin (MRSA).
An experimental study was performed in 45 mice of the Balb/c strain divided in nine groups of five individuals. Ten naive bacteriophages were isolated through clinical samples and hospital effluents. Lytic capacity and spectrum activity was evaluated on the basis of which six phages were selected for phagotherapy trials. Additionally, a commercial bacteriophage was used. The phagotherapy was evaluated through prophylaxis, and therapy of localized and systemic infections caused by MRSA by subcutaneous and intravenous inoculation, respectively. The effectiveness of three therapeutic schemes was tested: monotherapy, phage cocktail in multiple doses and phage cocktail in a single dose. The therapeutic activity of the phages was also compared with vancomycin and clindamycin.
The phage cocktail and the diverse dose therapy were effective in preventing and controlling MRSA localized infections; its activity was similar to the vancomycin and clindamycin activity. The single dose phage cocktail failed to control localized infection and phagotherapy was not effective in systemic infections.
Phagotherapy could be a viable alternative for infections caused by MRSA. Further studies that assess related aspects to phages and patient safety are required.
评估噬菌体对耐甲氧西林金黄色葡萄球菌（MRSA）引起的局部和全身感染的活性。
对45只Balb/c品系小鼠进行实验研究，分为9组，每组5只。通过临床样本和医院污水分离出10种天然噬菌体。根据裂解能力和光谱活性评估，选择6种噬菌体进行噬菌体疗法试验。此外，还使用了一种商业噬菌体。分别通过皮下和静脉接种，对MRSA引起的局部和全身感染进行预防和治疗，评估噬菌体疗法。测试了三种治疗方案的有效性：单一疗法、多剂量噬菌体鸡尾酒疗法和单剂量噬菌体鸡尾酒疗法。还将噬菌体的治疗活性与万古霉素和克林霉素进行了比较。
噬菌体鸡尾酒和不同剂量疗法在预防和控制MRSA局部感染方面有效；其活性与万古霉素和克林霉素的活性相似。单剂量噬菌体鸡尾酒未能控制局部感染，噬菌体疗法对全身感染无效。
噬菌体疗法可能是治疗MRSA感染的一种可行替代方法。需要进一步研究评估与噬菌体和患者安全相关的方面。
