Chhibber Sanjay, Kaur Tarsem, Kaur Sandeep
Department of Microbiology, Panjab University, Chandigarh, India.
J Basic Microbiol. 2014 Aug;54(8):775-80. doi: 10.1002/jobm.201300051. Epub 2013 May 20.
Phage therapy presents an alternative therapeutic option in treating infections caused by methicillin-resistant Staphylococcus aureus (MRSA) strains not responding to antibiotic therapy. However, it is essential to study the role of external factors that may influence the yield and potency of phage preparations intended for use in various in vitro and in vivo studies. The present study focuses on the effects of calcium in the entire infection process of a broad-spectrum lytic bacteriophage: MR-10. The presence of calcium increased the adsorption rate of the phage and also participated in the process of penetration of the phage genome into the host cytoplasm. A final concentration of 5 mM of calcium ions supplemented in soft agar during the phage titration process significantly increased the phage titer. Hence, incorporation of such divalent cations during the isolation of lytic phages active against MRSA strains and during the preparation of high-titer active phage preparations would definitely increase the isolation frequency and the final phage yield. This will contribute towards more effective phage preparations for use in treatments against MRSA infections.
噬菌体疗法为治疗对抗生素治疗无反应的耐甲氧西林金黄色葡萄球菌（MRSA）菌株引起的感染提供了一种替代治疗选择。然而，研究可能影响用于各种体外和体内研究的噬菌体制剂产量和效力的外部因素的作用至关重要。本研究聚焦于钙在广谱裂解性噬菌体MR-10整个感染过程中的作用。钙的存在提高了噬菌体的吸附率，并且还参与了噬菌体基因组进入宿主细胞质的过程。在噬菌体滴定过程中，在软琼脂中补充5 mM的最终钙离子浓度显著提高了噬菌体滴度。因此，在分离对MRSA菌株具有活性的裂解性噬菌体期间以及在制备高滴度活性噬菌体制剂期间加入这种二价阳离子肯定会提高分离频率和最终噬菌体产量。这将有助于制备更有效的噬菌体制剂用于治疗MRSA感染。
