Jiang Peiyue, Shen Kexin, Wang Xuerui, Song Haiqin, Yue Ying, Liu Tongjun
Department of Gynecological Oncology, First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
Department of Colorectal and Anal Surgery, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China.
Mol Med Rep. 2014 Jun;9(6):2347-51. doi: 10.3892/mmr.2014.2106. Epub 2014 Apr 2.
The targeting protein for the Xenopus kinesin-like protein 2 (TPX2), a microtubule-associated protein, has been utilized as a tool to evaluate, more precisely, the proliferative behavior of tumor cells. The abnormal expression of TPX2 in a variety of malignant tumor types has been reported, however less is known about its role in cervical cancer. In the present study, the association between TPX2 expression and the biological behavior of cervical cancer, was investigated. Immunohistochemistry and RT-PCR were used to detect the expression of TPX2 in cervical cancer tissues. The inhibitory effect of TPX2-siRNA on the growth of SiHa human cervical carcinoma cells was studied in vitro. TPX2 expression was identified as significantly higher in cervical carcinoma compared with the control, normal cervical tissues. TPX2 siRNA transfected into SiHa cells induced apoptosis and inhibited cell proliferation and invasion. Similar results were obtained by in vivo transplantation, as TPX2 siRNA transfection significantly reduced tumor growth of the xenograft in nude mice. The results demonstrated that TPX2 is important in the regulation of tumor growth in cervical cancer and therefore may be a potential therapeutic target as a novel treatment strategy.
非洲爪蟾驱动蛋白样蛋白2（TPX2）的靶向蛋白是一种微管相关蛋白，已被用作一种工具来更精确地评估肿瘤细胞的增殖行为。已有报道称TPX2在多种恶性肿瘤类型中表达异常，然而其在宫颈癌中的作用却知之甚少。在本研究中，我们调查了TPX2表达与宫颈癌生物学行为之间的关联。采用免疫组织化学和逆转录-聚合酶链反应（RT-PCR）检测宫颈癌组织中TPX2的表达。在体外研究了TPX2小干扰RNA（siRNA）对SiHa人宫颈癌细胞生长的抑制作用。结果表明，与对照正常宫颈组织相比，宫颈癌组织中TPX2表达显著更高。转染到SiHa细胞中的TPX2 siRNA可诱导细胞凋亡，并抑制细胞增殖和侵袭。通过体内移植也获得了类似结果，因为TPX2 siRNA转染显著降低了裸鼠体内异种移植瘤的生长。这些结果表明，TPX2在宫颈癌肿瘤生长调节中起重要作用，因此作为一种新的治疗策略，它可能是一个潜在的治疗靶点。
